Browsing by Author "Ajayi, Rachel F."

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    Gold nanoparticulate modified thioglycolic acid (tga) phenotype nanobiosensor for tuberculosis drugs
    (University of the Western Cape, 2022) Tshobeni, Ziyanda Zamaswazi; Ajayi, Rachel F.
    Tuberculosis (TB) carries on being a major global health issue and it is responsible for 1.5 million disease-related mortality in 2020, according to the World Health organisation (WHO). One of the major causes of death among TB patients is the development of drug-resistant TB due to the inappropriate dosage of the treatment drug, non-adherence to the prescribed regimen or using low quality medication. A fast and reliable detection method (such as biosensing) for real-time determination of a patient’s TB drug metabolism profile is therefore very important for ensuring appropriate prescription. In this study, an electrochemical nanobiosensor for the determination of the TB drug, isoniazid also known as isonicotinic acid hydrazide (INH) was developed. It consists of a cytochrome P450-2E1 (CYP2E1) isoenzyme covalently attached on a gold electrode (screen printed or disk) that was functionalized with thioglycolic acid-capped gold nanoparticle (TGA-AuNP), which was green-synthesized with banana peel extract (BPE) as the reducing agent.
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    Indium nanoparticulate-modified polyanilino-co-4-aminobenzoic acid amperometric sensor for the detection of lapatinib, a breast cancer drug.
    (University of the Western Cape, 2022) January, Jaymi Leigh; Ajayi, Rachel F.
    Lapatinib is a highly prescribed drug used in combination with other drugs to treat breast cancer. Due to drug toxicity-related side effects experienced by some patients, there is need to determine appropriate dosage for patients. A novel nanobiosensor for the determination of the biotransformation of a cancer drug (LAPA) was constructed with a co-polymer, poly(anilino-co-4-aminobenzoic acid) {poly(ANI-co-4-ABA)} coupled to ground coffee extract-based, green-synthesized indium nanoparticles (InNPs) to which cytochrome P450-3A4 (CYP3A4) enzyme was additionally attached onto a glassy carbon electrode. Polyaniline (PANI), 4-ABA, indium nanoparticles (InNPs), poly(ANI-co-4-ABA) and poly(ANI-co-4-ABA)/InNPs were synthesized and used as sensor materials. The x-ray diffraction (XRD) analysis indicated crystallinity (average crystallite size of 27.31 nm) and gave a sharp peak at 21.40 (211) that is indexed to indium metal.