Magister Scientiae Dentium - MSc(Dent) (Restorative Dentistry)
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Browsing by Author "Adam, Razia"
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Item An in vitro study on the effect of organic derived nano hydroxyapatite on dentine permeability(Universty of the Western Cape, 2023) Nyongesa, Christine Khanili; Adam, RaziaDentine sensitivity, hinders oral hygiene practices and limits consumption of specific foods and drinks. Studies have shown that, the severity of dentine sensitivity is related to the permeability of dentine tubules such that the more permeable the tubule is the higher the degree of sensitivity. Hydroxyapatite is one of the components known to block dentine tubules to reduce their permeability. The use of eggshells in deriving nano hydroxyapatite is an important approach as it also contributes to organic waste management. The purpose of this study was to assess and compare the effectiveness of organic nano hydroxyapatite derived from eggshells on dentine tubules permeability. This is an in vitro study, in which specimens of 72 human tooth dentine material were sectioned to thickness of 120, 250, 350, 500, 750, 1000 micrometers. The specimens were then exposed to four groups of treatment including deionized water, Sensodyne Sensitivity and Gum toothpaste, Colgate toothpaste and organic derived nanohydroxyapatite derived from eggshells.Item Microhardness and bleaching effectiveness on human tooth enamel after the application of nanohydroxyapatite(Universty of the Western Cape, 2023) Elmahdi, Elbara Elrayah; Adam, RaziaVarious bleaching agents, including hydrogen peroxide and carbamide peroxide, have been used in vital and non-vital bleaching. Nanohydroxyapatite synthesised using green methods are reported to possess bleaching potential. Although studies have indicated a reduction in enamel microhardness following bleaching, the correlation between bleaching and enamel hardness is inconsistent. This in vitro study aimed to investigate the potential of nanohydroxyapatite (nHA) as a bleaching agent and its effect on enamel microhardness on its own as well as in combination with carbamide peroxide (CP).Item Salivary carriage of candida species in children and their mothers in a Western Cape population salivary carriage of candida species in children and their mothers in a Western Cape population(Universty of the Western Cape, 2023) Layloo, Nazreen; Adam, RaziaCandida albicans has been detected together with Streptococcus mutans in high numbers in children with early childhood caries (ECC). The aim of this study was to explore the prevalence of Candida albicans in children with ECC in the Western Cape, and to explore the possibility of maternal relatedness. A cross-sectional study was conducted amongst 48 child-mother pairs within the Western Cape. DMFT/dmft scores for caries were assessed during a comprehensive oral examination. A structured questionnaire was administered to mothers of the participating children between the ages of 2 and 6 years. In addition to socio-demographic variables, dietary practices, antibiotic use, presence of an oral appliance, and frequency of dental visits were included. A single researcher conducted the clinical examination and sample collection. Saliva (2 mL) samples from all child-mother pairs were collected using sterile Eppendorf tubes. Saliva samples were cultured on both blood agar and Sabouraud dextrose agar plates. Cultures were isolated, counted, and identified. Positively identified cocci were then isolated and identified as specific Streptococcus species using the API strep 20E system (Biomerieux). Positive yeasts were sub-cultured and isolated on chromogenic agar plates and incubated at 37° C for 24 hours. Depending on the colour profile of the growth, the presence of Candida albicans was confirmed. Results were tabulated and statistical analyses conducted. Ethical approval was granted by the Biomedical Research and Ethics Committee of the University of the Western Cape (BM19/10/18).