Aptamer-driven biosensor technology for the quantitative analysis of C-reactive protein
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Date
2025
Journal Title
Journal ISSN
Volume Title
Publisher
John Wiley and Sons Inc
Abstract
C-reactive protein (CRP) is a widely recognized biomarker for early myocardial infarction (MI) detection, released into the bloodstream during heart inflammation. Traditional assays for CRP detection, like ELISA and immunoradiometric assays, are costly, time-consuming, and require large sample volumes. Aptasensors are becoming increasingly popular for MI diagnosis due to their affordability, simplicity, and potential for point-of-care use. In this study, an electrochemical aptasensor incorporating mercaptosuccinic acid-capped nickel selenide quantum dots (MSA-NiSe2 QD) were developed for CRP detection. The amine-modified aptamer was immobilized on the MSA-NiSe2 QD using EDC/NHS coupling chemistry. Chronocoulometric measurements showed high selectivity towards CRP in phosphate buffer, with a linear range of 10–110 pg/mL and a detection limit of 2.80 pg/mL. Cross-reactivity experiments confirmed the aptasensor's high selectivity for CRP. Testing in human serum samples demonstrated recovery rates of 94–100.5 %, indicating its suitability for clinical diagnostics. Validation studies with a commercial CRP ELISA kit showed the aptasensor's superior sensitivity in both physiological buffer and human serum.
Description
Keywords
C-reactive protein (CRP), Chronocoulometry (CC), Electrochemical aptasensor, Myocardial infarction (MI), Quantum dot (QD)
Citation
Oranzie, M., January, J.L., Sanga, N.A., Leve, Z.D., Mini, S., Cupido, C., Douman, S.F. and Iwuoha, E.I., 2025. Aptamer‐Driven Biosensor Technology for the Quantitative Analysis of C‐Reactive Protein. ChemElectroChem, 12(7), p.e202400667.