In-vitro study to investigate the antimicrobial and cytotoxic activity of a periodontal pocket gel: Pocket-X® gel By
| dc.contributor.advisor | Mulder van Staden, Sune | |
| dc.contributor.author | Moloi, Mpho | |
| dc.date.accessioned | 2025-02-20T09:20:14Z | |
| dc.date.available | 2025-02-20T09:20:14Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | Background. Periodontitis is a common oral disease resulting in periodontal breakdown and ultimately tooth loss. It is characterized by chronic inflammation of the periodontal tissues brought on by the formation and accumulation of substantial quantities of dental biofilm in a susceptible host. Numerous interventions are available for improving the management of periodontitis, including products such as the in situ gel Pocket-X® gel. There are limited studies assessing the efficacy of Pocket-X® gel, particularly in vitro to draw from. This study was aimed to evaluate the in vitro antimicrobial and potential cytotoxicity of Pocket-X® gel. Materials and methods. The antimicrobial efficacy of Pocket-X® gel was tested utilizing Aggregatibacter actinomycetemcomitans (A.a.), Porphyromonas gingivalis (P.g.) and Candida albicans (C.a.), which are pathogens commonly associated with the initiation and development of periodontitis. The zones of inhibition were used to measure the efficacy of Pocket-X® gel by well-diffusion method on Meuller-Hinton Agar over time. The potential cytotoxicity effect of Pocket-X® gel was assessed on buccal fibroblasts to simulate the oral tissue through the calculation of the percentage survival of buccal fibroblasts on tissue culture plates. Results. Pocket-X® gel demonstrated sustained antimicrobial effects over the 28 days test period across the three test organisms with zone of inhibition (ZOI) of 11.70(±0.78), 10.75(±0.68), and 11.86(±1.11) mm in diameter, respectively. | |
| dc.identifier.uri | https://hdl.handle.net/10566/20097 | |
| dc.language.iso | en | |
| dc.publisher | University of the Western Cape | |
| dc.subject | Pocket-X® gel | |
| dc.subject | Periodontitis | |
| dc.subject | In-vitro | |
| dc.subject | Antimicrobial effects | |
| dc.subject | Cytotoxic activity | |
| dc.title | In-vitro study to investigate the antimicrobial and cytotoxic activity of a periodontal pocket gel: Pocket-X® gel By | |
| dc.type | Article |