Analysis of the proteome of red and green leaf phenotypes of 'Bon Rouge' pear trees \trus communrls L. by 2-dimensional gel electrophoresis

dc.contributor.advisordu Preez, Marlene
dc.contributor.advisorNdimba, Bongani
dc.contributor.advisorRees, Jasper
dc.contributor.authorSehata, Majimi James
dc.date.accessioned2023-05-11T13:47:54Z
dc.date.accessioned2024-05-09T07:44:49Z
dc.date.available2023-05-11T13:47:54Z
dc.date.available2024-05-09T07:44:49Z
dc.date.issued2007
dc.description>Magister Scientiae - MScen_US
dc.description.abstractThe 'Bon Rouge' pear is a red pear cultivar derived from a rare, spontaneous bud mutation which occurred on the green pear 'William's Bon Chretien' (Bartlett). 'Bon Rouge' pear cultivar was observed to be reverting back to its original phenotypic green colour. To study the cellular changes occurring within the cells both the mutated red phenotype and the wild type green phenotype of 'Bon Rouge' were investigated using proteomics approach. The proteins from the tissues of interest were precipitated in the presence of l0o/o TCA and solubilized in urea/thiourea lysis buffer. The proteins were separated onl2Yo SDS-PAGE and2D PAGE in order to compare their protein expression profiles. The results from SDS-PAGE and 2D PAGE profiles show an elevated level of 53 kDa protein in green pears which was either absent or less expressed in red phenotype and this protein appears to be the only differentiating factor between the red and green 'Bon Rouge' phenotypes. This protein was subsequently identified by the MALDI-TOFMS to be large subunit of RuBisCO. Expression proteomics is a preferred method due to its reproducibility and it has a potential to provide direct identification of cultivars related proteins. Additionally, proteomics approaches could help the fruit crop gtowers andbreeders, to select and determine types of different cultivars at various growth stages.en_US
dc.identifier.urihttps://hdl.handle.net/10566/13205
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectProteomicsen_US
dc.subjectSDS-PAGEen_US
dc.subject2D-PAGEen_US
dc.subject'Bon Rouge'en_US
dc.subject'Bon Rouge' revertantsen_US
dc.subjectAnthocyaninsen_US
dc.subjectProteinsen_US
dc.subjectRuBisCOen_US
dc.subjectIEFen_US
dc.subjectMALDI-TOFen_US
dc.titleAnalysis of the proteome of red and green leaf phenotypes of 'Bon Rouge' pear trees \trus communrls L. by 2-dimensional gel electrophoresisen_US

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