Novel dyp-type peroxidases from actinobacteria: a genome mining approach
| dc.contributor.author | Groep, Juandré | |
| dc.date.accessioned | 2025-08-21T13:28:30Z | |
| dc.date.available | 2025-08-21T13:28:30Z | |
| dc.date.issued | 2024 | |
| dc.description.abstract | Twenty-one whole genome actinobacterial sequences were obtained from strains previously isolated from various environments. a genome-mining approach was applied to identify the presence of dye-decolourising (dyp-type or dyps) peroxidases. from genome sequence annotation twenty-six dyp-type sequences were identified and through bioinformatic analysis for the presence of twin-arginine translocation (tat) pathway signal peptides, was classified as belonging to class i (formerly class a) bacterial dyp-type peroxidases. phylogenetic analysis of the dyp sequences showed that the streptomyces derived dyps were predominantly grouping together and the nonstreptomyces derived dyps followed the same pattern. eight dyp-type peroxidases were selected for cloning, with the majority being derived from streptomyces spp., and primers were designed to amplify the dyp-type peroxidase genes. the pcr-amplicons were subjected to restriction digests and were ligated into pet20b(+). the constructs were transformed into escherichia coli jm109. only two of the constructs were successfully transformed: the dyp-type peroxidase genes from nocardia gamkensis czh20t and micromonospora sp. strain 30-1. further analysis using t7-primers confirmed sequence identity of the constructs. | |
| dc.identifier.uri | https://hdl.handle.net/10566/20792 | |
| dc.language.iso | en | |
| dc.publisher | University of the Western Cape | |
| dc.subject | Actinobacteria | |
| dc.subject | Peroxidase | |
| dc.subject | Dye-Decoloursing Peroxidase | |
| dc.subject | Dyp-Type | |
| dc.subject | Decolourisation | |
| dc.title | Novel dyp-type peroxidases from actinobacteria: a genome mining approach | |
| dc.type | Thesis |