Selecting an appropriate method for expressing S locus F-box-S2 recombinant protein
Loading...
Date
2017
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Elsevier
Abstract
A single locus (S locus) including at least two linked genes (female and male determinants) genetically controls
the gametophytic self-incompatibility (GSI) in apple, which has evolved to avoid self-fertilization. There has
been extensive work done on the female determinant of self-incompatibility, which has led to the determination
of the tertiary structure of S-RNase. However, the tertiary structure of male determinant (S locus F-box, SLF/SFB)
remains unresolved, which could mainly be due to difficulties associated with its expression in the recombinant
expression systems. In addressing this, we have evaluated several in vivo (prokaryotic and eukaryotic) and in vitro
expression systems for their efficiency in the expression of apple SLF2. The most successful expression of SLF2
(1 mg/ml) was achieved in E. coli using the synthesized gene in a high salt culture and applying heat shock
before induction of culture. We therefore present an approach for the efficient expression of S locus F-box
recombinant proteins for future functional and structural studies.
Description
Keywords
S locus F-box-S2, Recombinant protein, Protein expression
Citation
Ashkani, J. & D. J. G. Rees. (2017). Selecting an appropriate method for expressing S locus F-box-S2 recombinant protein. Biotechnology Reports, 15: 41–47