Development and validation of Non-CODIS miniSTR genotyping systems suitable for forensic case work in South Africa

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Date

2010

Journal Title

Journal ISSN

Volume Title

Publisher

University of the Western Cape

Abstract

The objective of this study was to develop and validate a six Non-CODIS miniSTR genotyping system and to determine its suitability for forensic casework in South Africa. In Non-CODIS miniSTR genotyping systems, smaller PCR products are amplified and the primers are positioned as close as possible to the repeat region. For this reason, these systems can be valuable in a variety of scenarios including complex paternity cases, missing persons work, and mass fatality disasters.

Description

Magister Scientiae - MSc

Keywords

Forensics, Mini short tandem repeats (miniSTRs), Loci, Non-CODIS (NC), Polymerase chain reaction (PCR), DNA sequencing, Degraded DNA, Internal validation, Population Studies, Allele frequencies, Forensic parameters

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