Bioassay-guided fractionation leads to the detection of cholic acid generated by the rare thalassomonas sp.

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dc.contributor.authorSchneider, Yannik K.-H.
dc.contributor.authorPheiffer, Fazlin
dc.contributor.authorHolst Hansen, Espen
dc.contributor.authorHammer Andersen, Jeanette
dc.date.accessioned2023-02-08T07:52:12Z
dc.date.available2023-02-08T07:52:12Z
dc.date.issued2023
dc.description.abstractBacterial symbionts of marine invertebrates are rich sources of novel, pharmaceutically relevant natural products that could become leads in combatting multidrug-resistant pathogens and treating disease. In this study, the bioactive potential of the marine invertebrate symbiont Thalassomonas actiniarum was investigated. Bioactivity screening of the strain revealed Gram-positive specific antibacterial activity as well as cytotoxic activity against a human melanoma cell line (A2058). The dereplication of the active fraction using HPLC-MS led to the isolation and structural elucidation of cholic acid and 3-oxo cholic acid. T. actiniarum is one of three type species belonging to the genus Thalassomonas. The ability to generate cholic acid was assessed for all three species using thin-layer chromatography and was confirmed by LC-MS. The re-sequencing of all three Thalassomonas type species using long-read Oxford Nanopore Technology (ONT) and Illumina data produced complete genomes, enabling the bioinformatic assessment of the ability of the strains to produce cholic acid. Although a complete biosynthetic pathway for cholic acid synthesis in this genus could not be determined based on sequence-based homology searches, the identification of putative penicillin or homoserine lactone acylases in all three species suggests a mechanism for the hydrolysis of conjugated bile acids present in the growth medium, resulting in the generation of cholic acid and 3-oxo cholic acid. With little known currently about the bioactivities of this genus, this study serves as the foundation for future investigations into their bioactive potential as well as the potential ecological role of bile acid transformation, sterol modification and quorum quenching by Thalassomonas sp. in the marine environmenten_US
dc.identifier.citationPheiffer,F . et al. (2023) .Bioassay-Guided Fractionation Leads to the Detection of Cholic Acid Generated by the Rare Thalassomonas sp. Mar. Drugs. 21, 2. https:// doi.org/10.3390/md21010002en_US
dc.identifier.urihttps:// doi.org/10.3390/md21010002
dc.identifier.urihttp://hdl.handle.net/10566/8379
dc.language.isoenen_US
dc.publisherPubmeden_US
dc.subjectThalassomonasen_US
dc.subjectBile aciden_US
dc.subjectCholic aciden_US
dc.subjectBioactivityen_US
dc.titleBioassay-guided fractionation leads to the detection of cholic acid generated by the rare thalassomonas sp.en_US
dc.typeArticleen_US

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