16 S rDNA primers and the unbiased assessment of thermophile diversity

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dc.contributor.authorBaker, Gillian
dc.contributor.authorCowan, Donald A.
dc.date.accessioned2010-09-21T11:46:07Z
dc.date.available2010-09-21T11:46:07Z
dc.date.issued2004
dc.description.abstractOur understanding of thermophile diversity is based predominantly on PCR studies of community DNA. ‘Universal’ and domain-specific rRNA gene PCR primers have historically been used for the assessment of microbial diversity without adequate regard to the degree of specificity of primer pairs to different prokaryotic groups. In a reassessment of the published primers commonly used for ‘universal’ and archaeal 16 S rDNA sequence amplification we note that substantial variations in specificity exist. An unconsidered choice of primers may therefore lead to significant bias in determination of microbial community composition. In particular, Archaea-specific primer sequences typically lack specificity for the Korarchaeota and Nanoarchaea and are often biased towards certain clades. New primer pairs specifically designed for ‘universal’ archaeal 16 S rDNA sequence amplification, with homology to all four archaeal groups, have been designed. Here we present the application of these new primers for preparation of 16 S libraries from thermophile communities.en_US
dc.identifier.citationBaker, G.C., Cowan, D.A. (2004). 16S rDNA primers and the unbiased assessment of thermophile diversity. Biochemical Society Transactions, 32:218-221
dc.identifier.urihttp://hdl.handle.net/10566/144
dc.inquiriesdcowan@uwc.ac.za
dc.language.isoenen_US
dc.privacy.showsubmittertrue
dc.publisherPortland Pressen_US
dc.rights© 2004 Biochemical Society. The Society permits author to deposit a post-print.
dc.source.urihttp://dx.doi.org/10.1007/s00792-008-0170-x
dc.status.ispeerreviewedtrue
dc.subjectMicrobial diversity assessmenten_US
dc.subjectUniversal primersen_US
dc.subjectArchaeaen_US
dc.subjectArchaea-specific primer sequencesen_US
dc.subjectKorarchaeotaen_US
dc.subjectNanoarchaeaen_US
dc.subjectPrimers for thermophile communitiesen_US
dc.title16 S rDNA primers and the unbiased assessment of thermophile diversityen_US
dc.typeArticleen_US

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