Bacterial morphotype grading for periodontal disease assessment

dc.contributor.authorSmeda-Pienaar, Kim
dc.contributor.authorKaambo, Eveline
dc.contributor.authorAfrica, Charlene W.J.
dc.date.accessioned2017-01-30T14:14:11Z
dc.date.available2017-01-30T14:14:11Z
dc.date.issued2017
dc.description.abstractBACKGROUND: Listgarten and Hellden (1978) used darkfield microscopy of wet mounts to differentiate between healthy and periodontally diseased sites in the mouth by expressing the different bacterial morphotypes observed as a percentage of the total number of bacteria counted. This method of periodontal disease assessment gained favour as a diagnostic tool but presented with the limitation of immediate examination to determine the number of motile rods present and an inability to distinguish between gingivitis and periodontitis. Grading of bacterial morphotypes into several distinct categories of health or disease (Ison and Hay, 2002), simplified the scoring system of Gram-stained smears for the diagnosis of bacterial vaginosis (Nugent et al. 1991). The application of a similar grading system using stained smears rather than wet mounts could be advantageous to the diagnosis of periodontal disease. OBJECTIVES/AIMS: This study tested the hypothesis that stained smears of dental plaque collected from the gingival crevice of individuals with varying probing pocket depths (PD) may provide a grading system for periodontal disease assessment. MATERIALS AND METHODS: Subgingival plaque samples were collected from 49 patients, stained with a silver stain and the proportions of each bacterial morphotype graded relative to their respective PD measurements. RESULTS: This technique allowed for a grading system of I–IV, with grade I indicating health and grade IV indicating severe periodontal disease. DISCUSSION: Stained smear examination eliminates the time restriction for motile rod enumeration and allows for storage of smears for future reference. CONCLUSION: Standardization of the microscopic areas to be evaluated or examined will facilitate the agreement of cut-off values for the diagnosis of periodontal disease.en_US
dc.description.sponsorshipThis material is based on work partially supported financially by the National Research Foundation (NRF) of South Africa.en_US
dc.identifier.citationSmeda-Pienaar K., et al. (2016). Bacterial morphotype grading for periodontal disease assessment. BDJ Open, 3: 16011en_US
dc.identifier.issn2056-807X
dc.identifier.urihttp://hdl.handle.net/10566/2508
dc.identifier.urihttp://dx.doi.org/10.1038/bdjopen.2016.11
dc.language.isoenen_US
dc.publisherNature Publishing Groupen_US
dc.relation.ispartofseries3;16011
dc.rightsCopyright, The Authors
dc.subjectPeriodontal diseaseen_US
dc.subjectDental plaqueen_US
dc.subjectBacterial morphotypeen_US
dc.titleBacterial morphotype grading for periodontal disease assessmenten_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Smeda_Bacterial_2017.pdf
Size:
335.88 KB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: