Electrochemical ochratoxin a immunosensors based on polyaniline nanocomposites templated with amine- and sulphate-functionalised polystyrene latex beads

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dc.contributor.advisorIwuoha, Emmanuel
dc.contributor.advisorJahed, Nazeem
dc.contributor.authorMuchindu, Munkombwe
dc.contributor.otherFaculty of Science
dc.date.accessioned2014-01-15T07:29:33Z
dc.date.accessioned2024-05-13T12:40:57Z
dc.date.available2011/05/30 12:48
dc.date.available2011/05/30
dc.date.available2014-01-15T07:29:33Z
dc.date.available2024-05-13T12:40:57Z
dc.date.issued2010
dc.descriptionPhilosophiae Doctor - PhDen_US
dc.description.abstractPolyaniline nanocomposites doped with poly(vinylsulphonate) (PV-SO3) and nanostructured polystyrene (PSNP) latex beads functionalized with amine (PSNP-NH2) and sulphate ((PSNP-OSO3) were prepared and characterised for use as nitrite electro-catalytic chemosensors and ochratoxin A immunosensors. The resultant polyaniline electrocatalytic chemosensors (PANI, PANI|PSNP-NH2 or PANI|PSNP-OSO3 −) were characterized by cyclic voltammetry (CV), ultraviolet-visible (UV-Vis) spectroscopy and scanning electron microscopy (SEM). Brown-Anson analysis of the multi-scan rate CV responses of the various PANI films gave surface concentrations in the order of 10−8 mol/cm. UV-vis spectra of the PANI films dissolved in dimethyl sulphoxide showed typical strong absorbance maxima at 480 and 740 nm associated with benzenoid p-p* transition and quinoid excitons of polyaniline, respectively. The SEM images of the PANI nanocomposite films showed cauliflower-like structures that were <100 nm in diameter. When applied as electrochemical nitrite sensors, sensitivity values of 60, 40 and 30 μA/mM with corresponding limits of detection of 7.4, 9.2 and 38.2 μM NO2 −, were obtained for electrodes, PANI|PSNP-NH2, PANI and PANI|PSNP-SO3 −; respectively. Immobilisation of ochratoxin A antibody onto PANI|PSNP-NH2, PANI and PANI|PSNPSO3 - resulted in the fabrication of immunosensors.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/14761
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectPolyanilineen_US
dc.subjectPoly (vinylsulphonate)en_US
dc.subjectPolystyreneen_US
dc.subjectNanocompositeen_US
dc.subjectNitriteen_US
dc.subjectOchratoxin A antigenen_US
dc.subjectOchratoxinen_US
dc.subjectA antibodyen_US
dc.subjectn-type semiconductoren_US
dc.subjectImmunosensoren_US
dc.subjectElectrochemical impedance spectroscopyen_US
dc.subjectEnzyme-linkeden_US
dc.subjectImmunosorbent assayen_US
dc.subjectCertified reference materialen_US
dc.subject2D gel electrophoresisen_US
dc.titleElectrochemical ochratoxin a immunosensors based on polyaniline nanocomposites templated with amine- and sulphate-functionalised polystyrene latex beadsen_US
dc.typeThesisen_US

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Philosophiae Doctor - PhD (Chemistry)