Factors influencing pigment production by halophilic bacteria and its effect on brine evaporation rates

Abstract

The disposal of reject brine, a highly concentrated waste by-product generated by various industrial processes, represents a major economic and environmental challenge. The common practice in dealing with the large amounts of brine generated is to dispose of it in a pond and allow it to evaporate. The rate of evaporation is therefore a key factor in the effectiveness of the management of these ponds. The addition of various dyes has previously been used as a method to increase the evaporation rate. In this study, a biological approach, using pigmented halophilic bacteria (as opposed to chemical dyes), was assessed. Two bacteria, an Arthrobacter sp. and a Planococcus sp. were selected due to their ability to increase the evaporation of synthetic brine. When using industrial brine, supplementation of the brine with an iron source was required to maintain the pigment production. Under these conditions, the Planococcus sp. CP5-4 produced a carotenoid-like pigment, which resulted in a 20% increase in the evaporation rate of the brine. Thus, the pigment production capability of halophilic bacteria could potentially be exploited as an effective step in the management of industrial reject brines, analogous to the crystallizer ponds used to mine salt from sea water.

Description

Keywords

Halophilic bacteria, Evaporation, Reject brine

Citation

Silva-Castro, G. A., Moyo, A. C., Khumalo, L., Zyl, L. J. V., Petrik, L. F., & Trindade, M. (2018). Factors influencing pigment production by halophilic bacteria and its effect on brine evaporation rates. Microbial Biotechnology, 12(2), 334–345. doi: 10.1111/1751-7915.13319