RAMICS: Trainable, high-speed and biologically relevant alignment of high-throughput sequencing reads to coding DNA
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Date
2014
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
Oxford University Press
Abstract
The challenge presented by high-throughput sequencing necessitates the development of novel
tools for accurate alignment of reads to reference sequences. Current approaches focus on using heuristics to map reads quickly to large genomes, rather
than generating highly accurate alignments in coding regions. Such approaches are, thus, unsuited for
applications such as amplicon-based analysis and
the realignment phase of exome sequencing and
RNA-seq, where accurate and biologically relevant
alignment of coding regions is critical. To facilitate
such analyses, we have developed a novel tool, RAMICS, that is tailored to mapping large numbers of sequence reads to short lengths (<10 000 bp) of coding
DNA.
Description
Keywords
Bioinfomatics, Coding, South Africa, Nucleic acids
Citation
Wright, I. A., & Travers, S. A. (2014). RAMICS: Trainable, high-speed and biologically relevant alignment of high-throughput sequencing reads to coding DNA. Nucleic Acids Research, 42 (13). 10.1093/nar/gku473