Trimetallic chalcogenide-sensitised interferon gamma aptasensor for tuberculosis

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dc.contributor.authorUhuo, Onyinyechi Vivian
dc.contributor.authorJanuarie, Kaylin Cleo
dc.contributor.authorMokwebo, Kefilwe Vanessa
dc.date.accessioned2025-11-03T07:35:36Z
dc.date.available2025-11-03T07:35:36Z
dc.date.issued2025
dc.description.abstractNovel copper indium tin sulfide nanomaterial synthesized with chitosan capping agent.•IFN-γ TB biomarker aptasensor was fabricated using a χt-c-CITS sensing platform.•By aptamer surface density study, χt-c-CITS enhanced aptamer loading by 84.6 %.•χt-c-CITS increased aptasensor signal by 73.9 % and target detection by 85.7 %.•"Turn-on" assay format was achieved and monitored by capacitive charge measurements. Tuberculosis (TB) is a highly contagious disease whose eradication has become challenging due to the difficulty of early and real-time diagnosis, especially in developing countries. These challenges arise due to the time-consuming, expensive, complicated, and non-user-friendly nature of the current diagnostic techniques, making them difficult to use except by a highly trained medical laboratory specialist. Since biosensors offer a faster, simpler, and highly sensitive alternative to traditional methods, there is a growing interest in enhancing biosensor signals for efficient application. One such method of biosensor signal amplification is the use of nanostructured materials. In this work, we report the positive effect of tuning the properties of a substituted-kesterite nanomaterial, copper indium tin sulfide (CITS), using a chitosan capping agent, for efficient signal amplification of interferon gamma (IFN-γ) TB biomarker detection. This nanostructured chitosan-capped copper indium tin sulfide (χt-c-CITS) nanomaterials served as an excellent sensing platform, improving aptamer loading by 84.6 % and signal response by 73.9 %. High sensitivity to changes in IFN-γ concentration was obtained with the χt-c-CITS-based aptasensor between 100 fM to 1 pM dynamic linear range, and a detection limit of 23.2 fM. Excellent electrochemical stability was achieved with 94 % and 95.6 % signal stability recorded after 20 repeated chronocoulometry measurements and a 32-day storage period, respectively. The aptasensor likewise showed excellent specificity and selectivity to IFN-γ in the presence of interfering agents, with potential application in real biological samples.
dc.identifier.citationUhuo, O. V. et al. (2025) Trimetallic chalcogenide-sensitised interferon gamma aptasensor for tuberculosis. Electrochimica acta. [Online] 538.
dc.identifier.urihttps://doi.org/10.1016/j.electacta.2025.146848
dc.identifier.urihttps://hdl.handle.net/10566/21355
dc.language.isoen
dc.publisherElsevier Ltd
dc.subjectAptasensor
dc.subjectChitosan
dc.subjectInterferon gamma
dc.subjectKesterite-based nanomaterials
dc.subjectTuberculosis
dc.titleTrimetallic chalcogenide-sensitised interferon gamma aptasensor for tuberculosis
dc.typeArticle

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