Inhibition of NOS- like activity in maize alters the expression of genes involved in H2O2 scavenging and glycine betaine biosynthesis

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dc.contributor.authorPhillips, Kyle
dc.contributor.authorMajola, Anelisa
dc.contributor.authorGokul, Arun
dc.contributor.authorKeyster, Marshall
dc.contributor.authorLudidi, Ndiko
dc.contributor.authorEgbichi, Ifeanyi
dc.date.accessioned2018-08-27T07:01:32Z
dc.date.available2018-08-27T07:01:32Z
dc.date.issued2018
dc.description.abstractNitric oxide synthase-like activity contributes to the production of nitric oxide in plants, which controls plant responses to stress. This study investigates if changes in ascorbate peroxidase enzymatic activity and glycine betaine content in response to inhibition of nitric oxide synthase-like activity are associated with transcriptional regulation by analyzing transcript levels of genes (betaine aldehyde dehydrogenase) involved in glycine betaine biosynthesis and those encoding antioxidant enzymes (ascorbate peroxidase and catalase) in leaves of maize seedlings treated with an inhibitor of nitric oxide synthase-like activity. In seedlings treated with a nitric oxide synthase inhibitor, transcript levels of betaine aldehyde dehydrogenase were decreased. In plants treated with the nitric oxide synthase inhibitor, the transcript levels of ascorbate peroxidase-encoding genes were down-regulated. We thus conclude that inhibition of nitric oxide synthase-like activity suppresses the expression of ascorbate peroxidase and betaine aldehyde dehydrogenase genes in maize leaves. Furthermore, catalase activity was suppressed in leaves of plants treated with nitric oxide synthase inhibitor; and this corresponded with the suppression of the expression of catalase genes. We further conclude that inhibition of nitric oxide synthase-like activity, which suppresses ascorbate peroxidase and catalase enzymatic activities, results in increased H2O2 content.en_US
dc.identifier.citationPhillips, K. et al. (2018). Inhibition of NOS- like activity in maize alters the expression of genes involved in H2O2 scavenging and glycine betaine biosynthesis. Scientific Reports, 8: 12628en_US
dc.identifier.issn2045-2322
dc.identifier.urihttp://dx.doi.org/10.1038/s41598-018-31131-z
dc.identifier.urihttp://hdl.handle.net/10566/3976
dc.language.isoenen_US
dc.privacy.showsubmitterFALSE
dc.publisherNature Publishing Groupen_US
dc.rightsScientific Reports is a fully open access journal.
dc.status.ispeerreviewedTRUE
dc.subjectNitric oxideen_US
dc.subjectPlantsen_US
dc.subjectStressen_US
dc.subjectAscorbate peroxidase enzymatic activityen_US
dc.subjectGlycine betaine contenten_US
dc.subjectH202en_US
dc.titleInhibition of NOS- like activity in maize alters the expression of genes involved in H2O2 scavenging and glycine betaine biosynthesisen_US
dc.typeArticleen_US

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