Ritalinic acid stimulates human sperm motility and maintains vitality in vitro
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Date
2020
Authors
Journal Title
Journal ISSN
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Publisher
Korean Society for Sexual Medicine and Andrology
Abstract
To evaluate the in vitro impact of ritalinic acid (RA), a major metabolite of methylphenidate (drug to treat attention-deficit hyperactivity disorder), on sperm motility, vitality and oxidative stress. Materials and Methods: Semen samples (n=13) were collected from healthy donors and a semen analysis was performed according to World Health Organization. Density gradient centrifugation was performed to isolate motile sperm. Samples were incubated with different concentrations (0, 1, 10, 100, and 1,000 ng/mL) of RA. The non-exposed group (0 ng/mL) was defined as the control group. Samples were analyzed for motility at different time points (0, 60, 150, 240, and 300 minutes) and for vitality and oxidation reduction potential (ORP) (at 0, 240, and 300 minutes). Sperm motility was assessed manually and motion kinetic parameters were recorded by computer aided semen analysis. Results: RA at any tested concentration significantly increased sperm motility compared to the control in a time-dependent manner with a maximum increase after 240 minutes. Motion kinetic parameters were not comparable.
Description
Keywords
Oxidative stress, Ritalinic acid, Sperm motility, Vitro, Density gradient centrifugation
Citation
Henkel, R. et al. (2020). Ritalinic acid stimulates human sperm motility and maintains vitality in vitro. World Journal of Men's Health ,38(1), 61-67