Isolation and characterization of strain exiguobacterium sp. Krl4, a producer of bioactive secondary metabolites from a Tibetan glacier
| dc.contributor.author | Tedesco, Pietro | |
| dc.contributor.author | Esposito, Fortunato Palma | |
| dc.contributor.author | van Zyl, Leonardo Joaquim | |
| dc.date.accessioned | 2022-02-17T09:48:03Z | |
| dc.date.available | 2022-02-17T09:48:03Z | |
| dc.date.issued | 2021 | |
| dc.description.abstract | Extremophilic microorganisms represent a unique source of novel natural products. Among them, cold adapted bacteria and particularly alpine microorganisms are still underexplored. Here, we describe the isolation and characterization of a novel Gram-positive, aerobic rod-shaped alpine bacterium (KRL4), isolated from sediments from the Karuola glacier in Tibet, China. Complete phenotypic analysis was performed revealing the great adaptability of the strain to a wide range of temperatures (5â40 âŚC), pHs (5.5â8.5), and salinities (0â15% w/v NaCl). Genome sequencing identified KRL4 as a member of the placeholder genus Exiguobacterium_A and annotation revealed that only half of the protein-encoding genes (1522 of 3079) could be assigned a putative function. An analysis of the secondary metabolite clusters revealed the presence of two uncharacterized phytoene synthase containing pathways and a novel siderophore pathway. Biological assays confirmed that the strain produces molecules with antioxidant and siderophore activities. Furthermore, intracellular extracts showed nematocidal activity towards C. elegans, suggesting that strain KRL4 is a source of anthelmintic compounds. | en_US |
| dc.identifier.citation | Tedesco, P. et al. (2021). Isolation and characterization of strain exiguobacterium sp. Krl4, a producer of bioactive secondary metabolites from a Tibetan glacier. Microorganisms, 9(5),890. https://doi.org/10.3390 /microorganisms9050890 | en_US |
| dc.identifier.issn | 2076-2607 | |
| dc.identifier.uri | https://doi.org/10.3390 /microorganisms9050890 | |
| dc.identifier.uri | http://hdl.handle.net/10566/7278 | |
| dc.language.iso | en | en_US |
| dc.publisher | MPDI | en_US |
| dc.subject | Cold-environment | en_US |
| dc.subject | Biosynthetic gene cluster | en_US |
| dc.subject | Genome mining | en_US |
| dc.subject | Tibetan glacier | en_US |
| dc.subject | Novel natural products | en_US |
| dc.title | Isolation and characterization of strain exiguobacterium sp. Krl4, a producer of bioactive secondary metabolites from a Tibetan glacier | en_US |
| dc.type | Article | en_US |