Identification of candidate plant growth promoting endophytes from Echium plantagineum roots
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Date
2018
Authors
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Publisher
University of the Western Cape
Abstract
The yearly increase of global population will result in a greater demand for crop
production, but with the climates changes and a lack of available agricultural land it will
become increasingly more difficult to provide sufficient crops to feed everyone
adequately. Application of the PGPE has proven over the past researches to be able
enhance growth of plants via various growth promoting mechanisms. To identify
suitable growth promoting bacteria candidate, E. plantagineum plant was used to
isolate endophytes from the root after surface sterilization. The isolates bacteria were
used to inoculate Brassica napus L seeds. The effects of isolate's ability to promote
growth were evaluated based on the certain growth parameters after 42 days in the
green house. Isolate CP5 produced highest results in all growth parameter. Isolates CP5
was selected as potential candidate as significant improvement was shown by this
isolate. This isolate was tested for the ability to produce ACC deaminase, solubilize
phosphate, synthesize IAA and siderophore production. Furthermore isolate CP5 growth
promotion abilities was tested on Brassica napus L under antimony stress.
Description
Magister Scientiae - MSc (Biotechnology)
Keywords
Brasscia napus L, plant growth promoting endophytic bacteria, endophytes, Echium plantagineum, ACC deaminase, Indole-3-acetic acid, phosphate solubilization, siderophore production, antimony