Effect of Cissampelos capensis rhizome extract on human spermatozoa in vitro

Simple item page
dc.contributor.authorShalaweh, Salem
dc.contributor.authorHenkel, Ralf
dc.contributor.authorErasmus, N.
dc.contributor.authorWeitz, Frans
dc.date.accessioned2016-06-01T08:39:47Z
dc.date.available2016-06-01T08:39:47Z
dc.date.issued2014
dc.description.abstractCissampelos capensis is commonly known by the Afrikaans name ‘dawidjies’ or ‘dawidjieswortel’. C. capensis is the most important and best-known medicinal plant of the family Menispermaceae used by the Khoisan and other rural people in the western regions of South Africa. Among numerous other ailments, it is traditionally taken to treat male fertility problems. Yet, no studies have investigated the effects of this plant or its extracts on human spermatozoa. The aim of study was to investigate the effects of C. capensis extracts on sperm function. A total of 77 semen samples were collected. Spermatozoa were washed with HTF-BSA medium and incubated with different concentrations of C. capensis (0, 0.05, 0.5, 5, 50, 200 μg ml−1) for 1 h at 37 °C. Sperm motility, vitality, acrosome reaction, reactive oxygen species (ROS), capacitation, Annexin V binding, DNA fragmentation and mitochondrial membrane potential (Δψm) were determined. While viability, Annexin V positivity and Δψm were not affected, the percentages of ROS-positive, TUNEL-positive, capacitated and hyperactivated spermatozoa increased significantly and dose-dependently. It is concluded that the alkaloids present in the extract of C. capansis rhizomes triggered sperm intrinsic superoxide production leading to sperm capacitation and DNA fragmentation.en_US
dc.description.accreditationWeb of Scienceen_US
dc.identifier.citationShalaweh SM, et al. (2014). Effect of cissampelos capensis rhizome extract on human spermatozoa in vitro. Andrologia, 47 (3): 1-10en_US
dc.identifier.issn0303-4569
dc.identifier.urihttp://hdl.handle.net/10566/2239
dc.identifier.urihttp://dx.doi.org/10.1111/and.12264
dc.language.isoenen_US
dc.privacy.showsubmitterfalse
dc.publisherWileyen_US
dc.source.urihttp://dx.doi.org/10.1111/and.12264
dc.status.ispeerreviewedtrue
dc.subjectAcrosome reactionen_US
dc.subjectCapacitationen_US
dc.subjectCissampelos capensisen_US
dc.subjectDNA fragmentationen_US
dc.subjectReactive oxygen speciesen_US
dc.titleEffect of Cissampelos capensis rhizome extract on human spermatozoa in vitroen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Shalaweh_Effect of_2014.pdf
Size:
250.94 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.55 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Research Articles (Medical Bioscience)