Establishing minion sequencing and genome assembly procedures for the analysis of the rooibos (aspalathus linearis) genome

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dc.contributor.authorMgwatyu, Yamkela
dc.contributor.authorCornelissen, Stephanie
dc.contributor.authorvan Heusden, Peter
dc.date.accessioned2022-09-20T09:43:31Z
dc.date.available2022-09-20T09:43:31Z
dc.date.issued2022
dc.description.abstractWhile plant genome analysis is gaining speed worldwide, few plant genomes have been sequenced and analyzed on the African continent. Yet, this information holds the potential to transform diverse industries as it unlocks medicinally and industrially relevant biosynthesis pathways for bioprospecting. Considering that South Africa is home to the highly diverse Cape Floristic Region, local establishment of methods for plant genome analysis is essential. Long-read sequencing is becoming standard procedure for plant genome research, as these reads can span repetitive regions of the DNA, substantially facilitating reassembly of a contiguous genome. With the MinION, Oxford Nanopore offers a cost-efficient sequencing method to generate long reads; however, DNA purification protocols must be adapted for each plant species to generate ultra-pure DNA, essential for these analyses.en_US
dc.identifier.citationMgwatyu, Y. et al. (2022). Establishing minion sequencing and genome assembly procedures for the analysis of the rooibos (aspalathus linearis) genome. Plants, 11(16), 2156. https://doi.org/10.3390/plants11162156en_US
dc.identifier.issn2223-7747
dc.identifier.urihttps://doi.org/10.3390/plants11162156
dc.identifier.urihttp://hdl.handle.net/10566/7926
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectRooibosen_US
dc.subjectPlant genomeen_US
dc.subjectBiotechnologyen_US
dc.subjectAfricaen_US
dc.subjectMedicinal plantsen_US
dc.titleEstablishing minion sequencing and genome assembly procedures for the analysis of the rooibos (aspalathus linearis) genomeen_US
dc.typeArticleen_US

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