Nanoparticulate of silver-modified poly (8-anilino-1-naphthalene sulphonic acid) nanobiosensor systems for the determination of Tuberculosis treatment drugs

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dc.contributor.advisorIwuoha, Emmanuel
dc.contributor.advisorBaker, Priscilla G.L.
dc.contributor.authorNgece, Rachel Fanelwa
dc.contributor.otherDept. of Chemistry
dc.contributor.otherFaculty of Science
dc.date.accessioned2013-07-30T11:35:04Z
dc.date.accessioned2024-05-13T12:40:59Z
dc.date.available2011/11/10 13:42
dc.date.available2011/11/10
dc.date.available2013-07-30T11:35:04Z
dc.date.available2024-05-13T12:40:59Z
dc.date.issued2011
dc.descriptionPhilosophiae Doctor - PhDen_US
dc.description.abstractThis study firstly reports the development and characterization of PVP-AgNPs, PANSA and PVPAgNPs/ PANSA nanocomposite on gold. AFM and TEM analyses revealed highly electroactive nanocomposites whose morphogy and properties were essential for the immobilization of CYP2E1. Secondly, the development and characterization of Au/PVPAgNPs/ PANSA/CYP2E1, Au/PVP-AgNPs/PANSA/SA-CYP2E1 and Au/PVPAgNPs/ PANSA/EG-CYP2E1 nanobiosensors are reported. AFM studies displayed globular morphologies with large roughness for the enzyme modified electrodes as opposed to those electrodes without enzymes. Finally, the biotransformation of standard solutions of TB drugs (isoniazid, ethambutol, pyrazinamide and rifampicin) in pH 7.4, 0.1 M phosphate buffer solution is reported. The biotransformations of the TB drugs were successfully studied using cyclic voltammetry (CV), square wave voltammetry (SWV), differential voltammetry (DPV) and steady state amperometry under aerobic conditions. Very good detection limits were obtained for the standard solutions of TB drugs and were found to be in the micromolar range. The detection limit values for the individual TB drugs were 0.55 μM (isoniazid), 0.7 μM (ethambutol), 0.054 μM (pyrazinamide) and 0.05 μM (rifampicin). The detection limit results showed that the nanobiosensors were more sensitive and suitable for the determination of the respective drugs in plasma and serum.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/14765
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectTuberculosisen_US
dc.subjectDOTS regimeen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectIsoniaziden_US
dc.subjectEthambutolen_US
dc.subjectPyrazinamideen_US
dc.subjectRifampicinen_US
dc.subjectPoly(8-anilino-1-naphthalene sulphonic acid)en_US
dc.subjectSilver nanoparticlesen_US
dc.subjectCytochrome P450-2E1en_US
dc.titleNanoparticulate of silver-modified poly (8-anilino-1-naphthalene sulphonic acid) nanobiosensor systems for the determination of Tuberculosis treatment drugsen_US
dc.typeThesisen_US

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Philosophiae Doctor - PhD (Chemistry)