Aqueous leaf extract of Sutherlandia frutescens attenuates ROS-induced apoptosis and loss of mitochondrial membrane potential in MPP+-treated SH-SY5Y cells

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dc.contributor.authorEkpo, E. O.
dc.contributor.authorEnogieru, Adaze Bijou
dc.contributor.authorOmoruyi, Sylvester Ifeanyi
dc.date.accessioned2021-01-08T06:58:49Z
dc.date.available2021-01-08T06:58:49Z
dc.date.issued2020
dc.description.abstractTo investigate the neuroprotective activity of the aqueous extract of Sutherlandia frutescens (SF) against 1-methyl-4-phenylpyridinium (MPP+)-induced toxicity in SH-SY5Y neuroblastoma cells. Methods: SH-SY5Y neuroblastoma cells were divided into different treatment groups: untreated cells, cells treated with MPP+ alone (2 mM), cells pretreated with SF (20 μg) prior to MPP+ (2 mM) treatment and cells treated with SF (20 μg) alone. Twenty-four hours after treatment with MPP+, cell viability was assessed by MTT assay, and changes in cell morphology, intracellular reactive oxygen species (ROS) production, mitochondrial membrane potential (MMP) as well as caspases 3/7 and 9 activities were determined. Results: Treatment of SH-SY5Y cells with MPP+ alone significantly altered cellular morphology, increased ROS production (p = 0.005), induced a significant loss of MMP (p = 0.0011) and caused significant apoptotic cell death, via the activation of caspases 3/7 and 9 (p ≤ 0.0359). These effects were however significantly (p ≤ 0.0359) attenuated in cells pre-treated with the aqueous leaf extract of SF, indicating the possible neuroprotective activity of the SF extract.en_US
dc.identifier.citationEkpo, E. O. et al. (2020). Aqueous leaf extract of Sutherlandia frutescens attenuates ROS-induced apoptosis and loss of mitochondrial membrane potential in MPP+-treated SH-SY5Y cells. Tropical Journal of Pharmaceutical Research, 19(3),549-555en_US
dc.identifier.issn1596-9827
dc.identifier.uri10.4314/tjpr.v19i3.13
dc.identifier.urihttp://hdl.handle.net/10566/5610
dc.language.isoenen_US
dc.publisherUniversity of Beninen_US
dc.subjectApoptosisen_US
dc.subjectNeurodegenerationen_US
dc.subjectParkinson's diseaseen_US
dc.subjectReactive oxygen speciesen_US
dc.subjectSutherlandia frutescensen_US
dc.titleAqueous leaf extract of Sutherlandia frutescens attenuates ROS-induced apoptosis and loss of mitochondrial membrane potential in MPP+-treated SH-SY5Y cellsen_US
dc.typeArticleen_US

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