Interaction analysis of statistically enriched mutations identified in Cameroon recombinant subtype CRF02_AG that can influence the development of Dolutegravir drug resistance mutations

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dc.contributor.authorMikas, Sello Given
dc.contributor.authorIsaacs, Darren
dc.contributor.authorChitongo, Rumbidzai
dc.date.accessioned2021-10-08T11:53:24Z
dc.date.available2021-10-08T11:53:24Z
dc.date.issued2021
dc.description.abstractThe Integrase (IN) strand transfer inhibitor (INSTI), Dolutegravir (DTG), has been given the green light to form part of first-line combination antiretroviral therapy (cART) by the World Health Organization (WHO). DTG containing regimens have shown a high genetic barrier against HIV-1 isolates carrying specific resistance mutations when compared with other class of regimens.We evaluated the HIV-1 CRF02_AG IN gene sequences from Cameroon for the presence of resistanceassociated mutations (RAMs) against INSTIs and naturally occurring polymorphisms (NOPs), using study sequences (n = 20) and (n = 287) sequences data derived from HIV Los Alamos National Laboratory database. The possible impact of NOPs on protein structure caused by HIV-1 CRF02_AG variations was addressed within the context of a 3D model of the HIV-1 IN complex and interaction analysis was performed using PyMol to validate DTG binding to the Wild type and seven mutant structures.en_US
dc.identifier.citationMikasi, S. G. et al. (2021). Interaction analysis of statistically enriched mutations identified in Cameroon recombinant subtype CRF02_AG that can influence the development of Dolutegravir drug resistance mutations. BMC Infectious Diseases, 21(1),379. https://doi.org/10.1186/s12879-021-06059-xen_US
dc.identifier.issn1471-2334
dc.identifier.urihttps://doi.org/10.1186/s12879-021-06059-x
dc.identifier.urihttp://hdl.handle.net/10566/6881
dc.language.isoenen_US
dc.publisherBMCen_US
dc.subjectCameroonen_US
dc.subjectMolecular modellingen_US
dc.subjectDolutegravir drugen_US
dc.subjectWorld Health Organization (WHO)en_US
dc.subjectHIV-1en_US
dc.titleInteraction analysis of statistically enriched mutations identified in Cameroon recombinant subtype CRF02_AG that can influence the development of Dolutegravir drug resistance mutationsen_US
dc.typeArticleen_US

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