Tandem lc-ms identification of antitubercular compounds in zones of growth inhibition produced by South African filamentous actinobacteria

Simple item page
dc.contributor.authorWatson, Daniel J.
dc.contributor.authorWiesner, Lubbe
dc.contributor.authorBeukes, Denzil
dc.date.accessioned2023-06-27T08:40:37Z
dc.date.available2023-06-27T08:40:37Z
dc.date.issued2023
dc.description.abstractNovel antitubercular compounds are urgently needed to combat drug-resistant Mycobacterium tuberculosis (Mtb). Filamentous actinobacteria have historically been an excellent source of antitubercular drugs. Despite this, drug discovery from these microorganisms has fallen out of favour due to the continual rediscovery of known compounds. To increase the chance of discovering novel antibiotics, biodiverse and rare strains should be prioritised. Subsequently, active samples need to be dereplicated as early as possible to focus efforts on truly novel compounds. In this study, 42 South African filamentous actinobacteria were screened for antimycobacterial activity using the agar overlay method against the Mtb indicator Mycolicibacterium aurum under six different nutrient growth conditions. Known compounds were subsequently identified through extraction and high-resolution mass spectrometric analysis of the zones of growth inhibition produced by active strains. This allowed the dereplication of 15 hits from six strains that were found to be producing puromycin, actinomycin D and valinomycin.en_US
dc.identifier.citationWatson, D. J. et al. (2023). Tandem lc-ms identification of antitubercular compounds in zones of growth inhibition produced by South African filamentous actinobacteria. Molecules, 28(11), 4276. https://doi.org/10.3390/molecules28114276en_US
dc.identifier.issn1420-3049
dc.identifier.urihttps://doi.org/10.3390/molecules28114276
dc.identifier.urihttp://hdl.handle.net/10566/9163
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectAntitubercularen_US
dc.subjectPharmaceuticalsen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectSouth Africaen_US
dc.subjectCovid-19en_US
dc.subjectPublic healthen_US
dc.titleTandem lc-ms identification of antitubercular compounds in zones of growth inhibition produced by South African filamentous actinobacteriaen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
watson_tandem lc-ms identification of antitubercular_2023.pdf
Size:
1.86 MB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Research Articles (School of Pharmacy)