Transcriptional regulatory networks in the mouse hippocampus

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dc.contributor.advisorBajic, Vladimir
dc.contributor.authorMacPherson, Cameron Ross
dc.date.accessioned2014-02-10T09:58:59Z
dc.date.accessioned2024-05-14T07:27:20Z
dc.date.available2009/12/04 14:52
dc.date.available2009/12/04
dc.date.available2014-02-10T09:58:59Z
dc.date.available2024-05-14T07:27:20Z
dc.date.issued2007
dc.descriptionMagister Scientiae - MSc
dc.description.abstractNeurological diseases are socially disabling and often mortal. To efficiently combat these diseases, a deep understanding of involved cellular processes, gene functions and anatomy is required. However, differential regulation of genes across anatomy is not sufficiently well understood. This study utilized large-scale gene expression data to define the regulatory networks of genes expressing in the hippocampus to which multiple disease pathologies may be associated. Specific aims were: ident i fy key regulatory transcription factors (TFs) responsible for observed gene expression patterns, reconstruct transcription regulatory networks, and prioritize likely TFs responsible for anatomically restricted gene expression. Most of the analysis was restricted to the CA3 sub-region of Ammon’s horn within the hippocampus. We identified 155 core genes expressing throughout the CA3 sub-region and predicted corresponding TF binding site (TFBS) distributions. Our analysis shows plausible transcription regulatory networks for twelve clusters of co-expressed genes. We demonstrate the validity of the predictions by re-clustering genes based on TFBS distributions and found that genes tend to be correctly assigned to groups of previously identified co-expressing genes with sensitivity of 67.74% and positive predictive value of 100%. Taken together, this study represents one of the first to merge anatomical architecture, expression profiles and transcription regulatory potential on such a large scale in hippocampal sub-anatomy.
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/14809
dc.language.isoen
dc.publisherUniversity of the Western Cape
dc.rights.holderUniversity of the Western Cape
dc.subjectBrain / Neuro-anatomy
dc.subjectHippocampus
dc.subjectAmmon's horn
dc.subjectNeurodegenerative disease
dc.subjectAlzheimer's disease
dc.subjectGene expression
dc.subjectTranscription regulation
dc.subjectRegulatory potential
dc.subjectTranscription factor
dc.subjectPromoter
dc.subjectTranscription factor binding site
dc.subjectTranscription regulatory network
dc.subjectAllen Brain Atlas
dc.titleTranscriptional regulatory networks in the mouse hippocampus
dc.typeThesis

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Magister Scientiae - MSc (Microbiology)