Optimisation of proteomics techniques for archival tumour blocks of a South African cohort of colorectal cancer

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dc.contributor.advisorChristoffels, Alan
dc.contributor.advisorRigby, Jonathan
dc.contributor.authorRossouw, Sophia Catherine
dc.date.accessioned2021-03-25T08:42:35Z
dc.date.accessioned2024-05-17T07:57:57Z
dc.date.available2022-01-01T22:10:06Z
dc.date.available2024-05-17T07:57:57Z
dc.date.issued2020
dc.descriptionPhilosophiae Doctor - PhDen_US
dc.description.abstractTumour-specific protein markers are usually present at elevated concentrations in patient biopsy tissue; therefore tumour tissue is an ideal biological material for studying cancer proteomics and biomarker discovery studies. To understand and elucidate cancer pathogenesis and its mechanisms at the molecular level, the collection and characterisation of a large number of individual patient tissue cohorts are required. Since most pathology institutes routinely preserve biopsy tissues by standardised methods of formalin fixation and paraffin embedment, these archived, FFPE tissues are important collections of pathology material, often accompanied by important metadata, such as patient medical history and treatments. FFPE tissue blocks are conveniently stored under ambient conditions for decades, while retaining cellular morphology due to the modifications induced by formalin.en_US
dc.identifier.urihttps://hdl.handle.net/10566/15287
dc.language.isoenen_US
dc.publisherUniversity of Western Capeen_US
dc.rights.holderUniversity of Western Capeen_US
dc.subjectFormalin-fixed paraffin-embedded (FFPE) proteomicsen_US
dc.subjectColorectal cancer (CRC)en_US
dc.subjectFFPE archival tissueen_US
dc.subjectProtein extraction protocolen_US
dc.subjectProtein purification methodsen_US
dc.titleOptimisation of proteomics techniques for archival tumour blocks of a South African cohort of colorectal canceren_US

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