PR-1-Like Protein as a Potential Target for the Identification of Fusarium oxysporum: An In Silico Approach

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dc.contributor.authorBakare, Olalekan Olanrewaju
dc.contributor.authorGokul, Arun
dc.contributor.authorKeyser, Marshall
dc.date.accessioned2021-09-13T11:25:27Z
dc.date.available2021-09-13T11:25:27Z
dc.date.issued2021-07
dc.description.abstractFusarium oxysporum remains one of the leading causes of economic losses and poor crop yields; its detection is strained due to its presentation in various morphological and physiological forms. This research work sought to identify novel biomarkers for the detection of Fusarium oxys porum using in silico approaches. Experimentally validated anti-Fusarium oxysporum antimicrobial peptides (AMPs) were used to construct a profile against Fusarium oxysporum. The performance and physicochemical parameters of these peptides were predicted. The gene for the Fusarium oxysporum receptor protein PR-1-like Protein, Fpr1, was identified and translated. The resulting protein model from the translation was then validated. The anti Fusarium oxysporum AMPs and Fusarium oxysporum receptor protein 3-D structures were characterized, and their docking interaction analyses were carried out. The HMMER in silico tool identified novel anti-Fusarium oxysporum antimicrobial peptides with good performance in terms of accuracy, sensitivity, and specificity. These AMPs also displayed good physicochemical properties and bound with greater affinity to Fusarium oxysporum protein receptor PR-1-like Protein. The tendency of these AMPs to precisely detect Fusarium oxysporum PR-1-like Protein, Fpr1, would justify their use for the identification of the fungus. This study would enhance and facilitate the identification of Fusarium oxysporum to reduce problems associated with poor crop yield, economic losses, and decreased nutritional values of plants to keep up with the growing population.en_US
dc.identifier.urihttps://doi.org/10.3390/biotech10020008
dc.identifier.urihttp://hdl.handle.net/10566/6653
dc.language.isoenen_US
dc.publisherBioTechen_US
dc.subjectantimicrobial peptidesen_US
dc.subjectpesticidesen_US
dc.subjectresistanceen_US
dc.subjectalgorithmsen_US
dc.subjectfungalen_US
dc.subjectproteinsen_US
dc.subjectreceptorsen_US
dc.titlePR-1-Like Protein as a Potential Target for the Identification of Fusarium oxysporum: An In Silico Approachen_US
dc.typeArticleen_US

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