Gonad differentiation in zebrafish is regulated by the canonical Wnt signalling pathway

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dc.contributor.authorSreenivasan, Rajini
dc.contributor.authorJiang, Junhai
dc.contributor.authorWang, Xingang
dc.contributor.authorBartfai, Richard
dc.contributor.authorKwan, Hsiao, Y.
dc.contributor.authorChristoffels, Alan
dc.contributor.authorOrban, Laszlo
dc.date.accessioned2015-06-18T15:07:38Z
dc.date.available2015-06-18T15:07:38Z
dc.date.issued2014
dc.description.abstractZebrafish males undergo a ‘‘juvenile ovary-to-testis’’ gonadal transformation process. Several genes, including nuclear receptor subfamily 5, group A (nr5a) and anti-Mu¨ llerian hormone (amh), and pathways such as Tp53-mediated germ-cell apoptosis have been implicated in zebrafish testis formation. However, our knowledge of the regulation of this complex process is incomplete, and much remains to be investigated about the molecular pathways and network of genes that control it. Using a microarray-based analysis of transforming zebrafish male gonads, we demonstrated that their transcriptomes undergo transition from an ovary-like pattern to an ovotestis to a testislike profile. Microarray results also validated the previous histological and immunohistochemical observation that there is high variation in the duration and extent of commitment to the juvenile ovary phase among individuals. Interestingly, global gene expression profiling of diverging zebrafish juvenile ovaries and transforming ovotestes revealed that some members of the canonical Wnt/beta-catenin signaling pathway were differentially expressed between these two phases. To investigate whether Wnt/beta-catenin signaling plays a role in zebrafish gonad differentiation, we used the Tg (hsp70l:dkk1b-GFP)w32 line to inhibit Wnt/beta-catenin signaling during gonad differentiation. Activation of dkk1b-GFP expression by heat shock resulted in an increased proportion of males and corresponding decrease in gonadal aromatase gene (cyp19a1a) expression. The Wnt target gene, lymphocyte enhancer binding factor 1 (lef1), was also down-regulated in the process. Together, these results provide the first functional evidence that, similarly to mammals, Wnt/beta-catenin signaling is a ‘‘pro-female’’ pathway that regulates gonad differentiation in zebrafish.en_US
dc.description.accreditationWeb of Scienceen_US
dc.identifier.citationSreenivasan, Rajini, et al. (2014). Gonad Differentiation in zebrafish is regulated by the canonical Wnt signaling Pathway. Biology of Reproduction, 90(2) 45,1-10
dc.identifier.issn1529-7268
dc.identifier.urihttp://hdl.handle.net/10566/1518
dc.language.isoenen_US
dc.privacy.showsubmitterfalse
dc.publisherSociety for the Study of Reproduction
dc.rights© 2014 by the Society for the Study of Reproduction, Inc. This is an Open Access article, freely available through Biology of Reproduction's Authors' Choice option.
dc.source.urihttp://dx.doi.org/10.1095/biolreprod.113.110874
dc.status.ispeerreviewedtrue
dc.subjectBeta-cataninen_US
dc.subjectDanio rerioen_US
dc.subjectSex differentiationen_US
dc.subjectZebrafishen_US
dc.subjectReproductionen_US
dc.titleGonad differentiation in zebrafish is regulated by the canonical Wnt signalling pathwayen_US
dc.typeArticleen_US

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