Carbon quantum dots as a co-reactant to Ru(bpy)32+ for electrochemiluminescence biosensing of cardiac troponin I

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Date

2025

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Journal ISSN

Volume Title

Publisher

Elsevier Ltd

Abstract

Carbon quantum dots are interesting materials for electrochemiluminescence as they can be used as luminophores and co-reactants. This research paper focuses on the use of carbon quantum dots as the co-reactant to Ru(bpy)32+ in an electrochemiluminescence system. Most of the work reported in literature applied the Ru(bpy)32+ /CQD ECL system for chemical sensors. In this work, we apply it to a biosensor for the detection of cardiac troponin I. The Carbon quantum dots were synthesized using a bottom-up method and were used to modify the surface of screen-printed electrodes for immobilization of the biomolecules. They were characterized by photoluminescence, ultraviolet-visible, Fourier transmission infrared spectroscopy and square wave voltammetry. When the carbon quantum dots were introduced to the system, an enhancement in the electrochemiluminescence intensity of Ru(bpy)32+ was observed. An electrochemiluminescence immunosensor was developed for the detection of cardiac troponin I. Electrochemiluminescence immunosensor achieved a low limit of detection of 0.02 ng/mL. The sensor also had a detection time of 15 min, allowing for rapid detections.

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Keywords

Carbon quantum dots, Cardiac troponin I, Co-reactants, Electrochemiluminescence, Biosensing

Citation

Louw, C.J., de Haan, P., Verpoorte, E. and Baker, P., 2025. Carbon quantum dots as a co-reactant to Ru (bpy) 32+ for electrochemiluminescence biosensing of cardiac troponin I. Electrochimica Acta, p.147516.