High-resolution insights into the in vitro developing blood-brain barrier: Novel morphological features of endothelial nanotube function

dc.contributor.authorMentor, Shireen
dc.contributor.authorFisher, David
dc.date.accessioned2021-10-14T15:15:01Z
dc.date.available2021-10-14T15:15:01Z
dc.date.issued2021
dc.description.abstractHigh-resolution electron microscopy (HREM) imaging of the in vitro blood-brain barrier (BBB), is a promising modality for investigating the dynamic morphological interplay underpinning BBB development. The successful establishment of BBB integrity is grounded in the brain endothelial cells (BEC’s) ability to occlude its paracellular spaces of brain capillaries through the expression of the intercellular tight junction (TJ) proteins. The impermeability of these paracellular spaces are crucial in the regulation of transcellular transport systems to achieve homeostasis of the central nervous system. To-date research describing morphologically, the dynamics by which TJ interaction is orchestrated to successfully construct a specialized barrier remains undescribed. In this study, the application of HREM illuminates the novel, dynamic and highly restrictive BEC paracellular pathway which is founded based on lateral membrane alignment which is the functional imperative for the mechanical juxtapositioning of TJ zones that underpin molecular bonding and sealing of the paracellular space.en_US
dc.identifier.citationMentor, S., & Fisher, D. (2021). High-resolution insights into the in vitro developing blood-brain barrier: Novel morphological features of endothelial nanotube function. Frontiers in Neuroanatomy, 15,661065. https://doi.org/10.3389/fnana.2021.661065en_US
dc.identifier.issn1662-5129
dc.identifier.urihttps://doi.org/10.3389/fnana.2021.661065
dc.identifier.urihttp://hdl.handle.net/10566/6915
dc.language.isoenen_US
dc.publisherFrontiers Mediaen_US
dc.subjectBlood-brain barrieren_US
dc.subjectNanovesiclesen_US
dc.subjectTunneling nanotubesen_US
dc.subjectTethering nanotubesen_US
dc.subjectHigh-resolution electron microscopyen_US
dc.titleHigh-resolution insights into the in vitro developing blood-brain barrier: Novel morphological features of endothelial nanotube functionen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
fnana-15-661065.pdf
Size:
7.95 MB
Format:
Adobe Portable Document Format
Description:
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description: