Gene expression alterations and molecular analysis of CHEK1 in solid tumors

dc.contributor.authorFadaka, Adewale Oluwaseun
dc.contributor.authorBakare, Olalekan Olanrewaju
dc.contributor.authorSibuyi, Nicole Remaliah Samantha
dc.date.accessioned2021-01-19T07:28:23Z
dc.date.available2021-01-19T07:28:23Z
dc.date.issued2020
dc.description.abstractAlterations in the Checkpoint kinase (CHEK1) gene, its regulation, and the possible clinical outcomes in human solid tumors have not been previously examined. Therefore, the present study was carried out to evaluate the expression of CHEK1 in solid tumors as well as the mechanism by which it can be regulated through non-coding RNAs. The expression of CHEK1 was investigated using Oncomine analysis. cBioPortal, Kaplan–Meier Plotter, and PrognoScan were performed to identify the prognostic roles of this gene in solid tumors. The copy number alteration, mutation, interactive analysis, and visualization of the altered networks were performed by cBioPortal. The molecular binding analysis was carried out by Schrodinger suite, PATCHDOCK, and discovery studio visualizer. The study demonstrated that the CHEK1 gene was differentially expressed in four different cancers, and that reduced CHEK1 mRNA expression is an unfavorable prognostic factor for patients with gastric and colorectal cancer. The molecular docking results showed that the CHEK1 gene can be regulated by microRNAs (miR-195-5p) due to the number of stable hydrogen atoms observed within the distance of 2.0 Å and the favorable amino acids (Ala221, Ile353, Ile365, Ile756, Val797, Val70, Val154, Ile159, Val347, Tyr804, Phe811, Tyr815, and Phe156) identified in the binding pocket of the argonaute protein.en_US
dc.identifier.citationFadaka, A. O. et al. (2020). Gene expression alterations and molecular analysis of CHEK1 in solid tumors. Cancers 12(3),662en_US
dc.identifier.issn2072-6694
dc.identifier.uri10.3390/cancers12030662
dc.identifier.urihttp://hdl.handle.net/10566/5672
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.subjectArgonaute proteinen_US
dc.subjectCanceren_US
dc.subjectCHEK1en_US
dc.subjectGene expressionen_US
dc.subjectMolecular dockingen_US
dc.titleGene expression alterations and molecular analysis of CHEK1 in solid tumorsen_US
dc.typeArticleen_US

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