Identification and characterisation of hemicellulases from thermophilic Actinomycetes

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dc.contributor.advisorCowan, Donald A.
dc.contributor.advisorBauer, Rolene
dc.contributor.advisorEaston, Samantha
dc.contributor.advisorTuffin, Marla
dc.contributor.authorMatthews, Lesley-Ann A.
dc.contributor.otherFaculty of Science
dc.date.accessioned2014-01-21T08:13:34Z
dc.date.accessioned2024-05-09T07:44:53Z
dc.date.available2011/06/01 09:54
dc.date.available2011/06/01
dc.date.available2014-01-21T08:13:34Z
dc.date.available2024-05-09T07:44:53Z
dc.date.issued2010
dc.descriptionMagister Scientiae - MScen_US
dc.description.abstractTo ensure the sustainability of bioethanol production, major attention has been directed to develop feedstocks which provide an alternative to food-crop biomass. Lignocellulosic (LC) biomass, which is chiefly composed of industrial plant residues, is a carbon-rich reservoir that is presently attracting much attention. However LC material is highly recalcitrant to bioprocessing and requires a mixture of physical and enzymatic pretreatment in order to liberate fermentable sugars. Thermostable enzymes are extremely desirable for use in thermophilic fermentations due to their inherent stability. Hemicellulose, a core constituent of LC, requires a cascade of hemicellulases to stimulate the depolymerisation of its xylan backbone. α-L-arabinofuranosidase (AFase) increases the rate of lignocellulose biodegradation by cleaving arabinofuranosyl residues from xylan thereby increasing the accessibility of other hemicellulases. Twenty thermophilic Actinomycete isolates were screened for AFase activity using pnp-arabinofuranoside as the substrate. Three strains (ORS #1, NDS #4 and WBDS #9) displayed significant AFase activity and were identified as Streptomyces species with 16S rRNA gene sequence analysis. Genomic DNA was isolated from these strains and a cosmid library constructed in the shuttle vector pDF666. Subsequent functional and PCR-based screening revealed no positive clones.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/13231
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectBioethanolen_US
dc.subjectLignocelluloseen_US
dc.subjectThermophilicen_US
dc.subjectStreptomycesen_US
dc.subjectSequencingen_US
dc.subjectPurificationen_US
dc.subjectα-L-arabinofuranosidaseen_US
dc.titleIdentification and characterisation of hemicellulases from thermophilic Actinomycetesen_US
dc.typeThesisen_US

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