Role of cytocentrifugation combined with nuclear fast picroindigocarmine staining in detecting cryptozoospermia in men diagnosed with azoospermia
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Date
2022
Authors
Journal Title
Journal ISSN
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Publisher
Seoul: Korean Society for Sexual Medicine and Andrology
Abstract
Azoospermia is defined as the absence of spermatozoa in the pellet of a centrifuged semen sample. In fact, when a
basic semen analysis fails to detect sperm in the ejaculate, there is still the possibility of detecting rare sperm after centrifugation of the sample and examination of the pellet. In this study, we assessed the role of Cytospin centrifugation in combination
with the nuclear fast picroindigocarmine (NF-PIC) staining in identifying sperm in azoospermic samples. Semen samples of 251 men diagnosed as having azoospermia after standard examination were further analyzed by Cytospin centrifugation in combination with NF-PIC staining.
Description
Keywords
Azoospermia, Centrifugation, Male infertility, Semen, Staining and labeling
Citation
Sharma, R. K. et al. (2022). Role of cytocentrifugation combined with nuclear fast picroindigocarmine staining in detecting cryptozoospermia in men diagnosed with azoospermia. The world journal of men's health. 10.5534/wjmh.210210