The paracrine effect of hypoxic and normoxic cancer secretion on the proliferation of brain endothelial cells (bend.3)
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Date
2022
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
: Background: This study aimed to investigate the disruption of cell cycle phases of bEnd.3
cells exposed to cancer paracrine secretion. Cancer cells have been reported to use the secretion
of paracrine factors to compromise the endothelial barrier to prepare for their passage into the
parenchyma. As cancer cells are known to act differently under conditions of hypoxia, we investigated
how conditional media (CM) derived from breast and glioblastoma cells incubated under conditions
of normoxia and hypoxia would affect proliferation of brain endothelial cells (bEnd.3). Methods:
Brain endothelial cells (bEnd.3) were cultivated with normoxic and hypoxic CM generated from
breast cancer MCF7 cells and glioblastoma U-87 cells. Cell proliferation was evaluated using the
trypan blue exclusion assay and phases of the cell cycle were evaluated using flow cytometry. Results:
bEnd.3 proliferations was suppressed more aggressively with hypoxic CM after 72 and 96 h; cell
cycle analysis showed that paracrine treatment tended to prevent BECs from entering the G2 phase,
thus suppressing cell division.
Description
Keywords
Cell proliferation, Brain endothelial cell, Hypoxia, Normoxia, Cancer
Citation
Rado, M., & Fisher, D. (2022). The paracrine effect of hypoxic and normoxic cancer secretion on the proliferation of brain endothelial cells (bend.3). Cells, 11(7), 1197. https://doi.org/10.3390/cells11071197