Rare fungal species isolated from Libyan diabetic patients

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dc.contributor.authorEsmaio, Mustafa
dc.contributor.authorAbrantes, Pedro Miguel dos Santos
dc.contributor.authorAfrica, Charlene W.J.
dc.date.accessioned2016-10-07T13:42:00Z
dc.date.available2016-10-07T13:42:00Z
dc.date.issued2016
dc.description.abstractBACKGROUND AND OBJECTIVES: The emerging resistance of Candida species to antifungals routinely used to treat candidiasis in HIV patients and in patients with diabetes mellitus (DM) has resulted in the frequent isolation of non-albicans Candida species. This study aimed to establish the prevalence and fluconazole resistance profiles of yeasts other than commonly identified Candida species which may be found colonizing the oral mucosa of Libyan patients with DM. METHODS: Fungal species were isolated from the oral cavity of DM-positive patients attending a diabetes clinic in Misrata Diabetes Centre in Libya. This study included patients aged between 35 and 95 years and excluded subjects who had been on antifungal therapy within two weeks prior to sample collection. The identification of the isolated species was done by growing the isolates on selective and chromogenic media and by API ID 32C biochemical testing. Antimicrobial susceptibility testing of the isolates to the antifungal fluconazole was performed using disk diffusion. The study complied with the Declaration of Helsinki (2013). RESULTS: Forty-four rare fungal isolates representing ten fungal species were identified from the oral mucosa of 194 patients, with 28.6% of rare Candida species demonstrating resistance to fluconazole. Saprochaete capitata and Cryptococcus humicola isolates demonstrated high levels of resistance to fluconazole, with other yeast species showing lower resistance levels. CONCLUSION: The methodologies used in this study allowed for the accurate identification of rare fungal species. The API 32 ID system was found to be a better identification method when compared to chromogenic media, as some species could not be identified with the latter. This study emphasizes the importance of accurate species identification and antifungal surveillance in patients with underlying chronic diseases such as DM who have higher morbidity and mortality rates due to less known and resistant fungal infections.en_US
dc.description.sponsorshipLibyan Governmenten_US
dc.identifier.citationEsmaio, M. et al. (2016). Rare fungal species isolated from Libyan diabetic patients. Poster presented at the 6th Infection Control Africa Network Congress, (25-28 September), Johannesburgen_US
dc.identifier.urihttp://hdl.handle.net/10566/2443
dc.language.isoenen_US
dc.publisherInfection Control Africa Network (ICAN)en_US
dc.rightsUniversity of the Western Cape
dc.subjectMycologyen_US
dc.subjectDrug resistanceen_US
dc.subjectCandidaen_US
dc.subjectHIV patientsen_US
dc.subjectMisrata Diabetes Centreen_US
dc.subjectLibyaen_US
dc.subjectDiabetes mellitus (DM)en_US
dc.titleRare fungal species isolated from Libyan diabetic patientsen_US
dc.typePresentationen_US

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