A fumonisins immunosensor based on polyanilino-carbon nanotubes doped with palladium telluride quantum dots
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
MDPI
Abstract
An impedimetric immunosensor for fumonisins was developed based on
poly(2,5-dimethoxyaniline)-multi-wall carbon nanotubes doped with palladium telluride
quantum dots onto a glassy carbon surface. The composite was assembled by a layer-by-layer
method to form a multilayer film of quantum dots (QDs) and poly(2,5-dimethoxyaniline)-
multi-wall carbon nanotubes (PDMA-MWCNT). Preparation of the electrochemical
immunosensor for fumonisins involved drop-coating of fumonisins antibody onto the
composite modified glassy carbon electrode. The electrochemical impedance spectroscopy
response of the FB1 immunosensor (GCE/PT-PDMA-MWCNT/anti-Fms-BSA) gave a linear
range of 7 to 49 ng L−1
and the corresponding sensitivity and detection limits were
0.0162 kΩ L ng−1
and 0.46 pg L−1
, respectively, hence the limit of detection of the
GCE/PT-PDMA-MWCNT immunosensor for fumonisins in corn certified material was
calculated to be 0.014 and 0.011 ppm for FB1, and FB2 and FB3, respectively. These results
are lower than those obtained by ELISA, a provisional maximum tolerable daily intake
(PMTDI) for fumonisins (the sum of FB1, FB2, and FB3) established by the Joint
FAO/WHO expert committee on food additives and contaminants of 2 μg kg−1
and the maximum level recommended by the U.S. Food and Drug Administration (FDA) for
protection of human consumption (2–4 mg L−1
).
Description
Keywords
Anti-fumonisins antibody, Impedimetric immunosensor, Palladium telluride quantum dots, Poly(2,5-dimethoxyaniline)-multi-wall carbon nanotubes, Electrochemical
impedance spectroscopy
Citation
Masikini, M. (2015). A fumonisins immunosensor based on polyanilino-carbon nanotubes doped with palladium telluride quantum dots. Sensors,15: 529-546