Development of electrochemical ZnSe Quantam dots biosensors for low-level detection of 17β-Estradiol estrogenic endocrine disrupting compound

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dc.contributor.advisorIwuoha, Emmanuel
dc.contributor.authorJijana, Abongile Nwabisa
dc.contributor.otherDept. of Chemistry
dc.contributor.otherFaculty of Science
dc.date.accessioned2013-10-24T07:32:41Z
dc.date.accessioned2024-05-09T10:50:09Z
dc.date.available2011/05/11 11:47
dc.date.available2011/05/11
dc.date.available2013-10-24T07:32:41Z
dc.date.available2024-05-09T10:50:09Z
dc.date.issued2010
dc.descriptionMagister Scientiae - MScen_US
dc.description.abstractThe main thesis hub was on development of two electrochemical biosensors for the determination of 17β-estradiol-estradiol: an estrogenic endocrine disrupting compound. Endocronology have significantly shown that the endocrine disruptors contribute tremendously to health problems encountered by living species today, problems such as breast cancer, reproductive abnormalities, a decline in male population most significant to aquatic vertebrates, reduced fertility and other infinite abnormalities recurring in the reproductive system of mostly male species. The first biosensor developed for the detection of 17β-estradiol-estradiol endocrine disrupting compound; consisted of an electro-active polymeric 3-mercaptoprorionic acid capped zinc selenide quantum dots cross linked to horseradish peroxidase (HRP) enzyme as a bio-recognition element. The second biosensor developed was comprised of cysteamine self assembled to gold electrode, with 3-mercaptopropionic acid capped zinc selenide quantum dots cross linked to cytochrome P450-3A4 (CYP3A4) enzyme in the presence of 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide hydrochloride and succinimide.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/14484
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectElectrochemical biosensorsen_US
dc.subjectCytochrome P450-3A4 (CYP3A4)en_US
dc.subjectHorseradish peroxidase (HRP)en_US
dc.subjectZnSe quantum dotsen_US
dc.subjectConducting polymersen_US
dc.subjectCyclic voltammetry (CV)en_US
dc.subjectDifferential-pulse voltammetry (DPV)en_US
dc.subject17- estradiolen_US
dc.subject17α-ethnylestradiolen_US
dc.subjectEstrogenic Endocrine Disrupting Compounds (e-EDC)en_US
dc.subjectMichaelis Menten constanten_US
dc.subjectHydroxylationen_US
dc.titleDevelopment of electrochemical ZnSe Quantam dots biosensors for low-level detection of 17β-Estradiol estrogenic endocrine disrupting compounden_US
dc.typeThesisen_US

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