Biodegradation of high-concentration isopropanol by a solvent-tolerant thermophile, Bacillus pallidus
| dc.contributor.author | Bustard, Mark T. | |
| dc.contributor.author | Whiting, Samantha | |
| dc.contributor.author | Cowan, Donald A. | |
| dc.contributor.author | Wright, Phillip C. | |
| dc.date.accessioned | 2010-10-05T14:41:27Z | |
| dc.date.available | 2010-10-05T14:41:27Z | |
| dc.date.issued | 2002 | |
| dc.description.abstract | The aerobic biodegradation of high-concentration, to 24 g l –1 , 2-propanol (IPA) by a thermophilic isolate ST3, identified as Bacillus pallidus , was successfully carried out for the first time. This solvent-tolerant B. pallidus utilized IPA as the sole carbon source within a minimal salts medium. Cultivation was carried out in 100-ml shake flasks at 60°C and compared with cultivation within a 1-l stirred tank reactor (STR). Specific growth rate () was about 0.2 h–1 for both systems, with a maximum cell density of 2.4 x 10 8 cells ml–1 obtained with STR cultivation. During exponential growth and stationary phase, IPA biodegradation rates were found to be 0.14 and 0.02 g l –1h–1, respectively, in shake-flask experiments, whereas corresponding values of 0.09 and 0.018 g l –1h–1 were achievable in the STR. Generation of acetone, the major intermediate in aerobic IPA biodegradation, was also monitored as an indicator of microbial IPA utilization. Acetone levels reached a maximum of 2.2–2.3 g l–1 after 72 and 58 h for 100-ml and 1-l systems, respectively. Both IPA and acetone were completely removed from the medium following 160 and 175 h, respectively, during STR growth, although this was not demonstrated within shake-flask reactions. Growth of B. pallidus on acetone or IPA alone demonstrated that the maximum growth rate () obtainable was 0.247 h–1 at 4 g l–1 acetone and 0.202 h–1 at 8 g l–1 IPA within shake-flask cultivation. These results indicate the potential of the solvent-tolerant thermophile B. pallidus ST3 in the bioremediation of hot solvent-containing industrial waste streams. | en_US |
| dc.identifier.citation | Bustard, M.T., Whiting, S., Cowan, D.A., Wright, P.C. (2002). Biodegradation of high concentration solvents by a solvent tolerant thermophile, Bacillus pallidus. Extremophiles, 6:319-323. | en_US |
| dc.identifier.uri | http://hdl.handle.net/10566/154 | |
| dc.inquiries | dcowan@uwc.ac.za | |
| dc.language.iso | en | en_US |
| dc.privacy.showsubmitter | true | |
| dc.publisher | Springer Verlag | en_US |
| dc.rights | Springer permits authors to deposit a post-print version of articles published by them in an open access repository. | |
| dc.source.uri | http://dx.doi.org/10.1007/s00792-001-0260-5 | |
| dc.status.ispeerreviewed | true | |
| dc.subject | Biodegradation | en_US |
| dc.subject | Bacillus pallidus | en_US |
| dc.subject | Isopropanol (IPA) | en_US |
| dc.subject | Solvent tolerant | en_US |
| dc.subject | Volatile organic compounds (VOCs) | en_US |
| dc.subject | Thermophilic | en_US |
| dc.title | Biodegradation of high-concentration isopropanol by a solvent-tolerant thermophile, Bacillus pallidus | en_US |
| dc.type | Article | en_US |