Biodegradation of high-concentration isopropanol by a solvent-tolerant thermophile, Bacillus pallidus

dc.contributor.authorBustard, Mark T.
dc.contributor.authorWhiting, Samantha
dc.contributor.authorCowan, Donald A.
dc.contributor.authorWright, Phillip C.
dc.date.accessioned2010-10-05T14:41:27Z
dc.date.available2010-10-05T14:41:27Z
dc.date.issued2002
dc.description.abstractThe aerobic biodegradation of high-concentration, to 24 g l –1 , 2-propanol (IPA) by a thermophilic isolate ST3, identified as Bacillus pallidus , was successfully carried out for the first time. This solvent-tolerant B. pallidus utilized IPA as the sole carbon source within a minimal salts medium. Cultivation was carried out in 100-ml shake flasks at 60°C and compared with cultivation within a 1-l stirred tank reactor (STR). Specific growth rate () was about 0.2 h–1 for both systems, with a maximum cell density of 2.4 x 10 8 cells ml–1 obtained with STR cultivation. During exponential growth and stationary phase, IPA biodegradation rates were found to be 0.14 and 0.02 g l –1h–1, respectively, in shake-flask experiments, whereas corresponding values of 0.09 and 0.018 g l –1h–1 were achievable in the STR. Generation of acetone, the major intermediate in aerobic IPA biodegradation, was also monitored as an indicator of microbial IPA utilization. Acetone levels reached a maximum of 2.2–2.3 g l–1 after 72 and 58 h for 100-ml and 1-l systems, respectively. Both IPA and acetone were completely removed from the medium following 160 and 175 h, respectively, during STR growth, although this was not demonstrated within shake-flask reactions. Growth of B. pallidus on acetone or IPA alone demonstrated that the maximum growth rate () obtainable was 0.247 h–1 at 4 g l–1 acetone and 0.202 h–1 at 8 g l–1 IPA within shake-flask cultivation. These results indicate the potential of the solvent-tolerant thermophile B. pallidus ST3 in the bioremediation of hot solvent-containing industrial waste streams.en_US
dc.identifier.citationBustard, M.T., Whiting, S., Cowan, D.A., Wright, P.C. (2002). Biodegradation of high concentration solvents by a solvent tolerant thermophile, Bacillus pallidus. Extremophiles, 6:319-323.en_US
dc.identifier.urihttp://hdl.handle.net/10566/154
dc.inquiriesdcowan@uwc.ac.za
dc.language.isoenen_US
dc.privacy.showsubmittertrue
dc.publisherSpringer Verlagen_US
dc.rightsSpringer permits authors to deposit a post-print version of articles published by them in an open access repository.
dc.source.urihttp://dx.doi.org/10.1007/s00792-001-0260-5
dc.status.ispeerreviewedtrue
dc.subjectBiodegradationen_US
dc.subjectBacillus pallidusen_US
dc.subjectIsopropanol (IPA)en_US
dc.subjectSolvent toleranten_US
dc.subjectVolatile organic compounds (VOCs)en_US
dc.subjectThermophilicen_US
dc.titleBiodegradation of high-concentration isopropanol by a solvent-tolerant thermophile, Bacillus pallidusen_US
dc.typeArticleen_US

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