The effect of storage time on the platelet concentration of Choukroun's platelet rich fibrin (PRF)

dc.contributor.advisorStephen, L.X.G.
dc.contributor.advisorHiss, D
dc.contributor.authorPeck, M. Thabit
dc.date.accessioned2016-09-20T08:09:01Z
dc.date.accessioned2024-04-16T13:23:38Z
dc.date.available2016-09-20T08:09:01Z
dc.date.available2024-04-16T13:23:38Z
dc.date.issued2011
dc.descriptionMagister Chirurgiae Dentium (MChD)en_US
dc.description.abstractWound healing is a complex process characterised by the repair and reconstitution of lost or damaged tissue. By the mid 1990s, several methods were proposed to enhance wound healing of surgical sites by introducing high concentrations of human platelets to these areas. In the early 21st century, Choukroun et al (2006b) introduced a new type of platelet concentrate that was devoid of any additives, and required no specialised equipment for its production. This concentrate was termed Platelet-rich fibrin (PRF) and although various aspects of this biomaterial had been studied, very little is currently known about its storage properties. Aim: To determine whether storage time had a significant effect on the platelet concentration of Choukroun’s PRF Method: A total of 30 patients were enrolled into the study. Three blood samples of 10ml each were drawn from each patient. Two of the blood samples (Group A and Group B) were centrifuged to form PRF. The third sample was used to measure the baseline blood platelet concentration and was therefore not centrifuged. After PRF had formed in both test groups, it was removed from the test tubes at 2 different times i.e. immediately after centrifuge (Group A) or after 60 min of storage in the blood collecting tube (Group B). The remaining blood was then tested for platelet concentration and compared to each other and the baseline reading. Results: 14 males and 16 females participated in the study (average age 41.7 years). A mean blood platelet concentration of 282.8 ± 58.3 × 109/L was recorded for the baseline reading. Group A had a mean blood platelet concentration 7.9 ± 3.03 × 109/L. Group B had a mean blood platelet concentration of 4.0 ± 1.93 × 109/L. A statistically significant difference was seen between Groups A and B (p < 0.0001). Conclusions: Storage time has a significant effect of the platelet concentration of PRF. If stored over a period of 60 min, the platelet concentration of PRF increases. Further research is required to determine whether this finding is clinically significant.en_US
dc.identifier.urihttps://hdl.handle.net/10566/11036
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectBlood collecting tubesen_US
dc.subjectPlatelet rich fibrinen_US
dc.subjectBlooden_US
dc.subjectFibrin glueen_US
dc.titleThe effect of storage time on the platelet concentration of Choukroun's platelet rich fibrin (PRF)en_US

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