Green method synthesised graphene-silver electrochemical nanobiosensors for ethambutol and pyrazinamide
Abstract
A novel nanobiosensor was constructed with graphene oxide (GO) sheets coupled to
pear extract-based green-synthesised silver nanoparticles (Ag-NPs) to which cytochrome P450-2D6
(CYP2D6) enzyme was attached. The biosensor was applied in the electrochemical detection of
the tuberculosis (TB) treatment drugs, ethambutol (EMB) and pyrazinamide (PZA). The surface
morphology of the green-synthesised nanocomposites was studied by performing High-Resolution
Transmission Electron Microscopy (HR-TEM) and High-Resolution Scanning Electron Microscopy
(HR-SEM). Fourier Transform Infrared Spectroscopy (FTIR) and Raman Spectroscopy were used
for structural analysis, while Ultraviolet Visible (UV-Vis) Spectroscopy was used in the optical
characterisation of the nanocomposite material. Electrochemical studies on glassy carbon electrode
(GCE), which were done by Cyclic Voltammetry (CV), showed that the GO|Ag-NPs||GCE electrode
was highly conductive, and thereby indicating its suitability as a platform for nanobiosensor
development. The non-toxic and low-cost green GO|Ag-NPs|CYP2D6||GCE nanobiosensor was used
to determine EMB and PZA. The very low limit of detection (LOD) values of the biosensor for
EMB (0.2962 × 10−2 nM, S/N = 3) and PZA (0.897 × 10−2 nM, S/N = 3) demonstrate that the green
nanobiosensor is more sensitive than other biosensors reported for EMB and PZA.
Description
Keywords
Ethambutol, Cytochrome P450-2D6, Graphene oxide, Pyrazinamide, Silver nanoparticles
Citation
AjayI et al. (2020). Green method synthesised graphene-silver electrochemical nanobiosensors for ethambutol and pyrazinamide. Processes, 8(7),879