Sequence analysis of an Archaeal virus isolated from a hypersaline lake in Inner Mongolia, China

Simple item page
dc.contributor.authorPagaling, Eulyn
dc.contributor.authorHaigh, Richard D.
dc.contributor.authorGrant, William D.
dc.contributor.authorCowan, Donald A.
dc.contributor.authorJones, Brian
dc.contributor.authorMa, Yanhe
dc.contributor.authorVentosa, Antonio
dc.contributor.authorHeaphy, Shaun
dc.date.accessioned2010-09-20T08:35:18Z
dc.date.available2010-09-20T08:35:18Z
dc.date.issued2007
dc.description.abstractBackground: We are profoundly ignorant about the diversity of viruses that infect the domain Archaea. Less than 100 have been identified and described and very few of these have had their genomic sequences determined. Here we report the genomic sequence of a previously undescribed archaeal virus. Results: Haloarchaeal strains with 16S rRNA gene sequences 98% identical to Halorubrum saccharovorum were isolated from a hypersaline lake in Inner Mongolia. Two lytic viruses infecting these were isolated from the lake water. The BJ1 virus is described in this paper. It has an icosahedral head and tail morphology and most likely a linear double stranded DNA genome exhibiting terminal redundancy. Its genome sequence has 42,271 base pairs with a GC content of ~65 mol%. The genome of BJ1 is predicted to encode 70 ORFs, including one for a tRNA. Fifty of the seventy ORFs had no identity to data base entries; twenty showed sequence identity matches to archaeal viruses and to haloarchaea. ORFs possibly coding for an origin of replication complex, integrase, helicase and structural capsid proteins were identified. Evidence for viral integration was obtained. Conclusion: The virus described here has a very low sequence identity to any previously described virus. Fifty of the seventy ORFs could not be annotated in any way based on amino acid identities with sequences already present in the databases. Determining functions for ORFs such as these is probably easier using a simple virus as a model system.en_US
dc.identifier.citationPagaling, E.,et al.(2007). Sequence analysis of an archaeal virus isolated from a hypersaline lake in Inner Mongolia, China. BMC Genomics, 8:410-416
dc.identifier.urihttp://hdl.handle.net/10566/140
dc.language.isoenen_US
dc.privacy.showsubmittertrue
dc.publisherBioMed Centralen_US
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.source.urihttp://dx.doi.org/10.1186/1471-2164-8-410
dc.status.ispeerreviewedtrue
dc.subjectArchaeal virusen_US
dc.subjectGenome sequencingen_US
dc.subjectHypersaline lakeen_US
dc.titleSequence analysis of an Archaeal virus isolated from a hypersaline lake in Inner Mongolia, Chinaen_US
dc.typeArticleen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
PagalingSequenceAnalysis2007.PDF
Size:
1.77 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.55 KB
Format:
Item-specific license agreed upon to submission
Description:
Download

Collections

Research Articles (IMBM)