Aspects of the gastrointestinal uptake and metabolism of luteolin derivatives from Artemisia afra aqueous extract (preclinical)

dc.contributor.authorMukinda, James Tshikosa
dc.contributor.otherSchool of Pharmacy
dc.contributor.otherFaculty of Community and Health Sciences
dc.date.accessioned2014-02-10T14:04:59Z
dc.date.accessioned2024-05-15T07:16:47Z
dc.date.available2011/05/31 06:58
dc.date.available2011/05/31
dc.date.available2014-02-10T14:04:59Z
dc.date.available2024-05-15T07:16:47Z
dc.date.issued2011
dc.descriptionPhilosophiae Doctor - PhD
dc.description.abstractThe aim of this study was to investigate the effect the plant matrix and the structure of the flavonoid (i.e. whether aglycone or glycoside) may have on the gastrointestinal uptake and metabolism of luteolin derivatives from Artemisia afra traditional plant medicine. Specifically, how these two factors influenced the intestinal uptake and disposition of luteolin derivatives in pure and in Artemisia afra plant extract forms were to be assessed by investigating the uptake and metabolism of the luteolin derivatives in human intestinal epithelial Caco-2 cells and the perfused rat intestinal loop. To realize this aim, the following were determined: (1) identification and characterization of major luteolin derivatives found in Artemisia afra, (2) the effect of the plant matrix on the uptake of luteolin derivatives in Artemisia afra aqueous-extract forms across the Caco-2 cell monolayer, (3) the effect of the plant matrix on the absorption and metabolism of luteolin derivatives in Artemisia afra aqueous-extract forms in the perfused rat small intestine, (4) the effect of gut contents on the uptake and metabolism of luteolin derivatives in intestinal loop and (5) the metabolic profiles of luteolin derivatives obtained for the pure solutions versus plant aqueous extract solutions in Caco-2 cells and the rat intestine.
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/15040
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectArtemisia afra
dc.subjectFlavonoids
dc.subjectLuteolin
dc.subjectAglycone form
dc.subjectCaco-2 cells
dc.subjectUptake/absorption
dc.subjectApparent permeability
dc.subjectG.i.t metabolism
dc.subjectBioavailability
dc.titleAspects of the gastrointestinal uptake and metabolism of luteolin derivatives from Artemisia afra aqueous extract (preclinical)en_US
dc.typeThesisen_US

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