An electrochemical DNA biosensor developed on a nanocomposite platform of gold and poly(propyleneimine) dendrimer

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dc.contributor.authorArotiba, Omotayo A.
dc.contributor.authorOwino, Joseph
dc.contributor.authorSonga, Everlyne
dc.contributor.authorHendricks, Nicolette
dc.contributor.authorWaryo, Tesfaye T.
dc.contributor.authorJahed, Nazeem
dc.contributor.authorBaker, Priscilla
dc.contributor.authorIwuoha, Emmanuel I.
dc.date.accessioned2016-12-21T12:25:01Z
dc.date.available2016-12-21T12:25:01Z
dc.date.issued2008
dc.description.abstractAn electrochemical DNA nanobiosensor was prepared by immobilization of a 20mer thiolated probe DNA on electro-deposited generation 4 (G4) poly(propyleneimine) dendrimer (PPI) doped with gold nanoparticles (AuNP) as platform, on a glassy carbon electrode (GCE). Field emission scanning electron microscopy results confirmed the codeposition of PPI (which was linked to the carbon electrode surface by C-N covalent bonds) and AuNP ca 60 nm. Voltammetric interrogations showed that the platform (GCE/PPI-AuNP) was conducting and exhibited reversible electrochemistry (E°′ = 235 mV) in pH 7.2 phosphate buffer saline solution (PBS) due to the PPI component. The redox chemistry of PPI was pH dependent and involves a two electron, one proton process, as interpreted from a 28 mV/pH value obtained from pH studies. The charge transfer resistance (Rct) from the electrochemical impedance spectroscopy (EIS) profiles of GCE/PPI-AuNP monitored with ferro/ferricyanide (Fe(CN)6 3-/4-) redox probe, decreased by 81% compared to bare GCE. The conductivity (in PBS) and reduced Rct (in Fe(CN)6 3-/4-) values confirmed PPI-AuNP as a suitable electron transfer mediator platform for voltammetric and impedimetric DNA biosensor. The DNA probe was effectively wired onto the GCE/PPI-AuNP via Au-S linkage and electrostatic interactions. The nanobiosensor responses to target DNA which gave a dynamic linear range of 0.01 - 5 nM in PBS was based on the changes in Rct values using Fe(CN)6 3-/4- redox probe.en_US
dc.identifier.citationOmotayo, A. et al. (2008). An electrochemical DNA biosensor developed on a nanocomposite platform of gold and poly(propyleneimine) dendrimer. Sensors, 8: 6791-6809en_US
dc.identifier.issn1424-8220
dc.identifier.urihttp://hdl.handle.net/10566/2474
dc.identifier.urihttp://dx.doi.org/10.3390/s8128262
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAll articles published by MDPI are made immediately available worldwide under an open access license.
dc.subjectPoly(propyleneimine) dendrimeren_US
dc.subjectGold nanoparticleen_US
dc.subjectElectrochemical impedance spectroscopyen_US
dc.subjectElectrochemical DNA biosensoren_US
dc.subjectDNAen_US
dc.titleAn electrochemical DNA biosensor developed on a nanocomposite platform of gold and poly(propyleneimine) dendrimeren_US
dc.typeArticleen_US

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