Improved cellulase expression in diploid yeast strains enhanced consolidated bioprocessing of pretreated corn residues

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Date

2019

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ScienceDirect

Abstract

In an effort to find a suitable genetic background for efficient cellulolytic secretion, genetically diverse strains were transformed to produce core fungal cellulases namely, β-glucosidase (BGLI), endoglucanase (EGII) and cellobiohydrolase (CBHI) in various combinations and expression configurations. The secreted enzyme activity levels, gene copy number, substrate specificities, as well as hydrolysis and fermentation yields of the transformants were analysed. The effectiveness of the partially cellulolytic yeast transformants to convert two different pre-treated corn residues, namely corn cob and corn husk was then explored.

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Keywords

Corn residues, Cellulases, Fermentation, Enzyme ratio, S. cerevisiae

Citation

Davison, S. A., Keller, N. T., van Zyl, W. H., & den Haan, R. (2019). Improved cellulase expression in diploid yeast strains enhanced consolidated bioprocessing of pretreated corn residues. Enzyme and microbial technology, 131, 109382. https://doi.org/10.1016/j.enzmictec.2019.109382

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http://hdl.handle.net/10566/8260

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Research Articles (Biotechnology)