Electrochemical Aptatoxisensor Responses on Nanocomposites containing electro-deposited silver nanoparticles on poly(propyleneimine) dendrimer for the detection of microcystin-lr in freshwater

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dc.contributor.advisorBilibana, Mawethu P.
dc.contributor.advisorWilliams, Avril R.
dc.date.accessioned2017-01-25T13:41:23Z
dc.date.available2017-01-25T13:41:23Z
dc.date.issued2016
dc.description.abstractA sensitive and reagentless electrochemical aptatoxisensor was developed on cobalt (II) salicylaldiimine metallodendrimer (SDD-Co(II)) doped with electro-synthesized silver nanoparticles (AgNPs) for microcystin-LR (L, L-leucine; R, L-arginine), or MC-LR, detection in the nanomolar range. The GCE|SDD-Co(II)|AgNPs aptatoxisensor was fabricated with 5' thiolated aptamer through self-assembly on the modified surface of the glassy carbon electrode (GCE) and the electronic response was measured using cyclic voltammetry (CV). Specific binding of MC-LR with the aptamer on GCE|SDD-Co(II)|AgNPs aptatoxisensor caused the formation of a complex that resulted in steric hindrance and electrostatic repulsion culminating in variation of the corresponding peak current of the electrochemical probe. The aptatoxisensor showed a linear response for MC-LR between 0.1 and 1.1 ug L-1 and the calculated limit of detection (LOD) was 0.04 ug L-1. In the detection of MC-LR in water samples, the aptatoxisensor proved to be highly sensitive and stable, performed well in the presence of interfering analog and was comparable to the conventional analytical techniques. The results demonstrate that the constructed MC-LR aptatoxisensor is a suitable device for routine quantification of MC-LR in freshwater and environmental samples.en_US
dc.identifier.citationBilibana, Mawethu P. et al.(2016). Electrochemical Aptatoxisensor Responses on Nanocomposites containing electro-deposited silver nanoparticles on poly(propyleneimine) dendrimer for the detection of microcystin-lr in freshwater. Sensors, 16: 1901en_US
dc.identifier.issn1424-8220
dc.identifier.urihttp://hdl.handle.net/10566/2501
dc.identifier.urihttp://dx.doi.org/10.3390/s16111901
dc.language.isoenen_US
dc.publisherMDPIen_US
dc.rightsAll articles published by MDPI are made immediately available worldwide under an open access license
dc.subjectAptameren_US
dc.subjectAptatoxisensoren_US
dc.subjectCyanotoxinsen_US
dc.subjectElectrochemical biosensoren_US
dc.subjectMetallodendrimeren_US
dc.subjectMicrocystin-LRen_US
dc.subjectNanosensoren_US
dc.titleElectrochemical Aptatoxisensor Responses on Nanocomposites containing electro-deposited silver nanoparticles on poly(propyleneimine) dendrimer for the detection of microcystin-lr in freshwateren_US
dc.typeArticleen_US

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