Electrochemical cytochrome P450 enzymatic biosensors for the determination of the reactivity of TB drugs

Simple item page
dc.contributor.advisorIwuoha, Emmanuel
dc.contributor.advisorAjayi, Fanelwa
dc.contributor.authorRassie, Candice
dc.date.accessioned2020-04-16T13:00:33Z
dc.date.accessioned2024-05-13T12:40:44Z
dc.date.available2024-05-13T12:40:44Z
dc.date.issued2020
dc.descriptionPhilosophiae Doctor - PhDen_US
dc.description.abstractTuberculosis (TB) remains a global epidemic despite the fact that treatment has been available since the 1950’s. This disease is highly contagious and spreads via transmission of the Mycobacterium Tuberculosis (MTB) tubercle via coughing, sneezing and spitting. The disease has various side effects including weight loss, fatigue and even death. To date no cure has been found for TB and thus optimisation of treatment is a constant focus in health related research. TB is highly prevalent in South Africa due to the increased level of patients who are co-infected with HIV. Treatment for TB consists of first line drugs including isoniazid (INH), ethambutol (ETH), pyrazinamide (PYR) and rifampicin (RIF). These drugs are highly effective but also produce many adverse drug reactions (ADR’s) over the 6-month course of treatment. These reactions lead to patients not completing the course, losing quality of life and ultimately adding to the development of drug resistant strains of TB. A method of minimising these ADR’s is the development of a phenotype sensor, which is able to determine the metabolic profile of patients. Metabolic profiles play a huge role in the efficacy of treatment by tailoring treatment in order for patients to stay within the therapeutic range of treatment. This would in turn minimise both toxicity and ineffective treatment. Various methods for the quantification of drugs have been developed such as high performance liquid chromatography (HPLC), mass spectrometry (MS) and ultra-violet visible spectroscopy (UV-vis).en_US
dc.description.embargo2023-12-01
dc.identifier.urihttps://hdl.handle.net/10566/14730
dc.language.isoenen_US
dc.publisherUniversity of Western Capeen_US
dc.rights.holderUniversity of Western Capeen_US
dc.subjectBiosensoren_US
dc.subjectCytochrome P450en_US
dc.subjectCYP3A4en_US
dc.subjectDrug Metabolismen_US
dc.subjectEthambutolen_US
dc.subjectIsoniaziden_US
dc.subjectMetallodendrimeren_US
dc.subjectPhenotypeen_US
dc.subjectPolypropyleneimineen_US
dc.subjectPyrazinamideen_US
dc.subjectReal Samplesen_US
dc.subjectRifampicinen_US
dc.subjectTuberculosisen_US
dc.titleElectrochemical cytochrome P450 enzymatic biosensors for the determination of the reactivity of TB drugsen_US

Files

Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Rassie_Phd_NSC_2019.pdf
Size:
6.33 MB
Format:
Adobe Portable Document Format
Description:
Thesis
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Plain Text
Description:
Download

Collections

Philosophiae Doctor - PhD (Chemistry)