Alteration of Bax-to-Bcl-2 ratio modulates the anticancer activity of methanolic extract of Commelina benghalensis (Commelinaceae) in Jurkat T cells

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dc.contributor.authorMbazima, Vusi G.
dc.contributor.authorMokgotho, Matlou P.
dc.contributor.authorFebruary, Faghri
dc.contributor.authorRees, Jasper G.
dc.contributor.authorMampuru, Leseilane J.
dc.date.accessioned2016-06-14T13:47:08Z
dc.date.available2016-06-14T13:47:08Z
dc.date.issued2008
dc.description.abstractStem extracts of Commelina benghalensis (Linn.), although not extensively documented, are frequently used in traditional medicine for the treatment of ailments such as skin malformations and outgrowths. Accordingly, the study was aimed to investigate possible molecular mechanisms that are associated with the potential anti-carcinogenic property of this agrofield weed. Jurkat T cells were exposed to different concentrations (0-600 ug/ml) of the crude methanolic extract of C. benghalensis to evaluate their growth inhibitory and apoptosis inducing effects. The extract elicited a dose- and time-dependent inhibition of cell proliferation, followed by a concomitant decrease in cell viability. The observed cytotoxicity was linked to the induction of apoptosis as determined by morphological and biochemical features known to be associated with the advent of apoptosis. Real time quantitative RT-PCR and Western blot analyses of Bax, Bcl-2 and p53 exhibited aberrant expression profiles of these genes under various treatment conditions. Taken together, the data suggest that the crude methanolic extract of C. benghalensis contains bioactive compounds that may be beneficial in the treatment of malignant growths, and that this apparent antineoplastic activity is a consequence of dysregulated expression of apoptosis-responsive genes. These observations could provide a credible scientific justification upon which the ethnopharmacological utilisation of C. benghalensis is founded.en_US
dc.identifier.citationMbazima, V.G. et al. (2008). Alteration of Bax-to-Bcl-2 ratio modulates the anticancer activity of methanolic extract of Commelina benghalensis (Commelinaceae) in Jurkat T cells. African Journal of Biotechnology, 7(20): 3569-3576en_US
dc.identifier.issn1684-5315
dc.identifier.urihttp://hdl.handle.net/10566/2322
dc.language.isoenen_US
dc.privacy.showsubmitterFALSE
dc.publisherAcademic Journalsen_US
dc.rights.uriAcademic Journals strongly supports the Open Access initiative. Abstracts and full texts of all articles published by Academic Journals are freely accessible to everyone immediately after publication.
dc.source.urihttp://dx.doi.org/10.5897/AJB08.492
dc.status.ispeerreviewedTRUE
dc.subjectApoptosisen_US
dc.subjectBcl-2en_US
dc.subjectBaxen_US
dc.subjectCommelina benghalensisen_US
dc.subjectJurkat T cellsen_US
dc.subjectTraditional medicineen_US
dc.titleAlteration of Bax-to-Bcl-2 ratio modulates the anticancer activity of methanolic extract of Commelina benghalensis (Commelinaceae) in Jurkat T cellsen_US
dc.typeArticleen_US

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