The identification of novel regulatory elements in the promoters of heat shock response genes

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dc.contributor.advisorMeyer, Mervin
dc.contributor.authorNcube, Sifelani
dc.contributor.otherDept. of Biotechnology
dc.contributor.otherFaculty of Science
dc.date.accessioned2013-07-31T07:20:25Z
dc.date.accessioned2024-05-09T07:44:57Z
dc.date.available2011/11/07 08:47
dc.date.available2011/11/07
dc.date.available2013-07-31T07:20:25Z
dc.date.available2024-05-09T07:44:57Z
dc.date.issued2010
dc.descriptionMasters of Scienceen_US
dc.description.abstractThe main objective of this study was to investigate promoter sequences of putative HSR genes for the presence of unique regulatory elements and modules that might be involved in the regulation of HSR. In order to achieve this objective, an in silico promoter analysis strategy was devised, which focused on the identification of promoter sequences and regulatory elements, and modelling of promoter modules by using Genomatix software tools such as MatInspector and ModelInspector. Results showed that two modules (EGRF_SP1F_01 and SP1F_CEBP_01) were conserved in the promoter sequences of three well-known Hsp-genes (Hsp90, Hsp105β and αβ-crystallin). Screening the 60 target gene promoters for the presence of the two modules revealed that 12 genes (20 %) contained both modules. These included Moesin, Proline-4 hydroxylase, Poly(A) binding protein and Formin-binding protein. None of these genes had been previously associated with heat shock response.en_US
dc.description.countrySouth Africa
dc.identifier.urihttps://hdl.handle.net/10566/13251
dc.language.isoenen_US
dc.publisherUniversity of the Western Capeen_US
dc.rights.holderUniversity of the Western Capeen_US
dc.subjectApoptosisen_US
dc.subjectCardiomyocytesen_US
dc.subjectCytoprotectionen_US
dc.subjectHeat shock proteinsen_US
dc.subjectHeat shock responseen_US
dc.subjectInducible gene expressionen_US
dc.subjectMolecular chaperonesen_US
dc.subjectReal-time quantitative PCRen_US
dc.subjectRegulatory elementsen_US
dc.subjectUnfolded protein responseen_US
dc.titleThe identification of novel regulatory elements in the promoters of heat shock response genesen_US
dc.typeThesisen_US

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