Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase
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Date
2009
Authors
Journal Title
Journal ISSN
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Publisher
University of the Western Cape
Abstract
In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction chromatography. Amidase coupled enzyme stop assays were conducted using 3-cyanopyridine as a substrate, whereas continuous enzyme kinetics were conducted using acrylonitrile as a substrate.
Description
Magister Scientiae - MSc
Keywords
Nitrogen compounds, Nitriles, Nitrogen