Screening for subtate tolerant Geobacillus pallidus RAPc8 nitrile hydratase

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Date

2009

Journal Title

Journal ISSN

Volume Title

Publisher

University of the Western Cape

Abstract

In this study G. pallidus RAPc8 NHase mutants were screened for reduced substrate inhibition compared to the wild type enzyme. Wild type and mutant enzymes were expressed and purified using hydrophobic interaction chromatography. Amidase coupled enzyme stop assays were conducted using 3-cyanopyridine as a substrate, whereas continuous enzyme kinetics were conducted using acrylonitrile as a substrate.

Description

Magister Scientiae - MSc

Keywords

Nitrogen compounds, Nitriles, Nitrogen

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